Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Damuni, Z.; Reed, L.J.
    Purification and characterization of a divalent cation-independent, spermine-stimulated protein phosphatase from bovine kidney mitochondria (1987), J. Biol. Chem., 262, 5133-5138.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 acetyl-CoA
-
 Bos taurus
3.1.3.16 ATP
-
 Bos taurus
3.1.3.16 dephospho-CoA
-
 Bos taurus
3.1.3.16 diphosphate
-
 Bos taurus
3.1.3.16 F-
-
 Bos taurus
3.1.3.16 guanosine 5'-(beta,gamma-imido)triphosphate
-
 Bos taurus
3.1.3.16 isobutyryl-CoA
-
 Bos taurus
3.1.3.16 isovaleryl-CoA
-
 Bos taurus
3.1.3.16 Nucleoside mono-, di-, and triphosphates e.g. GTP, GDP, ATP, ADP Bos taurus
3.1.3.16 phosphate
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.16 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.16 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.3.16 -20°C, 50 mM imidazole chloride buffer, pH 7.3, 1 mM EDTA, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM dithiothreitol, 1.4 M NaCl, 10% glycerol, 8 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 phosphocasein + H2O
-
 Bos taurus casein + phosphate
-
 ?
3.1.3.16 phosphoproteins + H2O
-
 Bos taurus proteins + phosphate
-
 ?
3.1.3.16 phosphorylase a + H2O
-
 Bos taurus phosphorylase b + phosphate
-
 ?
3.1.3.16 phosphorylated BCKDH complex + H2O i.e. branched-chain alpha-keto acid dehydrogenase, phosphorylated Bos taurus BCKDH complex + phosphate
-
 ?
3.1.3.16 phosphorylated pyruvate dehydrogenase + H2O
-
 Bos taurus pyruvate dehydrogenase + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.16 dimer phosphatase SP Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 6.9 7.1
-
 Bos taurus
3.1.3.16 7.6 8 presence of spermine Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.16 6.4 7.6 inactive above in absence of spermine Bos taurus