Any feedback?
Literature summary extracted from
- Properties of the smooth muscle phosphatase from turkey gizzard (1985), Adv. Protein Phosphatases, 1, 367-382.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.16 | ATP | phosphatases SMP-I and SMP-II | Meleagris gallopavo |  |
| 3.1.3.16 | diphosphate | - |
Meleagris gallopavo | |
| 3.1.3.16 | F- | phosphatase SMP-II | Meleagris gallopavo | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.16 | 0.0015 | 0.0095 | myosin light chain | - |
Meleagris gallopavo | |
| 3.1.3.16 | 0.0059 | - |
heavy meromyosin | - |
Meleagris gallopavo |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.16 | Mg2+ | - |
Meleagris gallopavo | |
| 3.1.3.16 | Mn2+ | activation | Meleagris gallopavo | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.16 | 150000 | 165000 | phosphatase SMP-I, sedimentation equilibrium centrifugation | Meleagris gallopavo |
| 3.1.3.16 | 220000 | 230000 | phosphatase SMP-I, gel filtration | Meleagris gallopavo |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.16 | phosphoprotein + H2O | Meleagris gallopavo | role of phosphatases in smooth muscle contractile systems | protein + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.16 | Meleagris gallopavo | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.16 | 3 forms | Meleagris gallopavo |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.16 | gizzard | - |
Meleagris gallopavo | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.16 | heavy meromyosin + H2O | - |
Meleagris gallopavo | ? | - |
? | |
| 3.1.3.16 | phosphohistone + H2O | - |
Meleagris gallopavo | histone + phosphate | - |
? | |
| 3.1.3.16 | phosphomyosin light chain + H2O | - |
Meleagris gallopavo | myosin light chain + phosphate | - |
? | |
| 3.1.3.16 | phosphoprotein + H2O | role of phosphatases in smooth muscle contractile systems | Meleagris gallopavo | protein + phosphate | - |
? | |
| 3.1.3.16 | phosphoproteins + H2O | - |
Meleagris gallopavo | proteins + phosphate | - |
? | |
| 3.1.3.16 | phosphorylase a + H2O | - |
Meleagris gallopavo | phosphorylase b + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.16 | dimer | AB, phosphatase 2B, i.e. calcineurin, A: 61000, catalytic subunit, calmodulin and cation binding, SDS-PAGE | Meleagris gallopavo |
| 3.1.3.16 | dimer | phosphatase SMP-IV | Meleagris gallopavo |
| 3.1.3.16 | trimer | phosphatase SMP-I | Meleagris gallopavo |
| 3.1.3.16 | trimer | alpha: 32000-38000, beta: 60000-69000, gamma: 54000-57000, delta: 74000 | Meleagris gallopavo |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.16 | 7 | 7.5 | phosphatase SMP-IV | Meleagris gallopavo |
| 3.1.3.16 | 7.5 | - |
phosphatase SMP-II | Meleagris gallopavo |
| 3.1.3.16 | 8 | - |
phosphatase SMP-I | Meleagris gallopavo |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
