Literature summary extracted from
Levy, C.C.; Goldman, P.
Residue specificity of a ribonuclease which hydrolyzes polycytidylic acid (1970), J. Biol. Chem., 245, 3257-3262.
Application
EC Number |
Application |
Comment |
Organism |
---|
4.6.1.21 |
analysis |
- |
Enterobacter sp. |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.6.1.21 |
EDTA |
inhibition after enzyme dialysis against 0.001 M EDTA |
Enterobacter sp. |
|
4.6.1.21 |
o-phenanthroline |
inhibition after enzyme dialysis against 0.001 M o-phenanthroline |
Enterobacter sp. |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.6.1.21 |
Cd2+ |
reverses EDTA- and o-phenanthroline-induced inhibition |
Enterobacter sp. |
|
4.6.1.21 |
Co2+ |
reverses EDTA- and o-phenanthroline-induced inhibition |
Enterobacter sp. |
|
4.6.1.21 |
Mg2+ |
reverses EDTA- and o-phenanthroline-induced inhibition |
Enterobacter sp. |
|
4.6.1.21 |
Mn2+ |
reverses EDTA- and o-phenanthroline-induced inhibition |
Enterobacter sp. |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.6.1.21 |
RNA + H2O |
Enterobacter sp. |
preferential cleavage at CpA |
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.21 |
Enterobacter sp. |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.6.1.21 |
- |
Enterobacter sp. |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
4.6.1.21 |
-15°C, stable, withstands freezing and thawing |
Enterobacter sp. |
4.6.1.21 |
-196°C, stable, withstands freezing and thawing |
Enterobacter sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.6.1.21 |
RNA + H2O |
preferential cleavage at CpA |
Enterobacter sp. |
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates |
- |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.6.1.21 |
7 |
8 |
- |
Enterobacter sp. |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.6.1.21 |
5.5 |
- |
no activity below, poly(C) |
Enterobacter sp. |