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Literature summary extracted from

  • Boix, E.; Wu, Y.; Vasandani, V.M.; Saxena, S.K.; Ardelt, W.; Ladner, J.; Youle, R.J.
    Role of the N terminus in RNase A homologues: differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity (1996), J. Mol. Biol., 257, 992-1007.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.18 NaCl
-
 Homo sapiens
4.6.1.18 NaCl
-
 Bos taurus
4.6.1.18 NaCl
-
 Lithobates pipiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression in Escherichia coli Homo sapiens
4.6.1.18 onconase/RNase A hybrid protein, expression in Escherichia coli Lithobates pipiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.18 NaCl
-
 Bos taurus
4.6.1.18 NaCl
-
 Homo sapiens
4.6.1.18 NaCl
-
 Lithobates pipiens
4.6.1.18 Polyanions natural and synthetic, free poly(A), poly(U) Bos taurus
4.6.1.18 ribonuclease inhibitor
-
 Bos taurus
4.6.1.18 ribonuclease inhibitor
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.18 RNA + H2O Homo sapiens endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O Bos taurus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O Lithobates pipiens endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
-
-
4.6.1.18 Homo sapiens
-
-
-
4.6.1.18 Lithobates pipiens
-
 frog, mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18 of the recombinant and mutant protein Lithobates pipiens
4.6.1.18 of the recombinant protein Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 egg
-
 Lithobates pipiens
-
4.6.1.18 pancreas
-
 Homo sapiens
-
4.6.1.18 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 CpA + H2O
-
 Bos taurus adenosine + 3'-CMP
-
 ?
4.6.1.18 CpA + H2O
-
 Lithobates pipiens adenosine + 3'-CMP
-
 ?
4.6.1.18 poly (C) + H2O
-
 Homo sapiens 3'-CMP + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 poly (C) + H2O
-
 Bos taurus 3'-CMP + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 poly (C) + H2O
-
 Lithobates pipiens 3'-CMP + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 poly U + H2O
-
 Homo sapiens 3'-UMP + 3'-oligonucleotides
-
 ?
4.6.1.18 poly U + H2O
-
 Lithobates pipiens 3'-UMP + 3'-oligonucleotides
-
 ?
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Homo sapiens 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Lithobates pipiens 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 tRNA + H2O onconase, similar enzyme Lithobates pipiens 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 UpG + H2O
-
 Bos taurus guanosine + 3'-UMP
-
 ?
4.6.1.18 UpG + H2O
-
 Lithobates pipiens guanosine + 3'-UMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 onconase
-
 Lithobates pipiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.18 5.5
-
-
 Lithobates pipiens