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Literature summary extracted from

  • Weickmann, J.L.; Elson, M.; Glitz, D.G.
    Purification and characterization of human pancreatic ribonuclease (1981), Biochemistry, 20, 1272-1278.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.18 NaCl
-
 Bos taurus
4.6.1.18 NaCl at 0.1-0.25 M Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.18 iodoacetate
-
 Homo sapiens
4.6.1.18 NaCl
-
 Bos taurus
4.6.1.18 NaCl above 0.25 M Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.18 0.46
-
 cyclic 2',3'-CMP
-
 Bos taurus
4.6.1.18 1
-
 cyclic 2',3'-CMP
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.18 Na+ NaCl: 0.1-0.25 M optimum concentration Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.18 15000
-
-
 Bos taurus
4.6.1.18 15000
-
 pancreas, gel filtration, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.18 RNA + H2O Homo sapiens endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O Bos taurus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
-
-
4.6.1.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Homo sapiens
-
4.6.1.18 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.18 1100
-
 brain, poly(C) Bos taurus
4.6.1.18 2400
-
 brain, RNA Bos taurus
4.6.1.18 10300
-
 RNA Homo sapiens
4.6.1.18 11300
-
 pancreas, RNA Bos taurus
4.6.1.18 22600
-
 pancreas, poly(C) Bos taurus
4.6.1.18 35000
-
 poly(C) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Homo sapiens 3'-phosphomononucleotides
-
 ir
4.6.1.18 cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Bos taurus 3'-phosphomononucleotides
-
 ir
4.6.1.18 poly (C) + H2O
-
 Homo sapiens 3'-CMP + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 poly (C) + H2O
-
 Bos taurus 3'-CMP + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Homo sapiens 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O first step of hydrolysis is reversible Homo sapiens cyclic 2',3'-nucleoside monophosphate
-
 r
4.6.1.18 RNA + H2O first step of hydrolysis is reversible Bos taurus cyclic 2',3'-nucleoside monophosphate
-
 r

Subunits

EC Number Subunits Comment Organism
4.6.1.18 monomer
-
 Bos taurus
4.6.1.18 monomer 1 * 15000, pancreas, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.18 8.2
-
-
 Homo sapiens