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Literature summary extracted from
- Purification and properties of two ribonucleases and a nuclease from barley seeds (1980), Biochim. Biophys. Acta, 614, 102-112.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.2 | Co2+ | slight | Hordeum vulgare |  |
| 3.1.30.2 | Cu2+ | - |
Hordeum vulgare | |
| 3.1.30.2 | DNA | inhibits activity against RNA | Hordeum vulgare | |
| 3.1.30.2 | Fe2+ | slight | Hordeum vulgare | |
| 3.1.30.2 | Hg2+ | - |
Hordeum vulgare | |
| 3.1.30.2 | Mn2+ | slight | Hordeum vulgare | |
| 3.1.30.2 | Zn2+ | slight | Hordeum vulgare | |
| 4.6.1.19 | Cu2+ | - |
Hordeum vulgare | |
| 4.6.1.19 | Fe2+ | slight | Hordeum vulgare | |
| 4.6.1.19 | Hg2+ | - |
Hordeum vulgare | |
| 4.6.1.19 | Mn2+ | slight | Hordeum vulgare | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.2 | Zn2+ | inhibition | Hordeum vulgare | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.2 | 37000 | - |
gel filtration | Hordeum vulgare |
| 4.6.1.19 | 19000 | - |
gel filtration | Hordeum vulgare |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.2 | Hordeum vulgare | - |
barley | - |
| 4.6.1.19 | Hordeum vulgare | - |
barley, 2 forms with different pH-optima: RNAase I, RNAase II | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.2 | - |
Hordeum vulgare |
| 4.6.1.19 | barley | Hordeum vulgare |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.30.2 | seed | - |
Hordeum vulgare | - |
| 4.6.1.19 | seed | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.2 | additional information | - |
- |
Hordeum vulgare |
| 4.6.1.19 | additional information | - |
- |
Hordeum vulgare |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.2 | DNA + H2O | hydrolysis rate about 3 times more slowly than compared to RNA | Hordeum vulgare | 5'-phosphonucleotides + 5'-phosphomononucleotides | - |
? | |
| 3.1.30.2 | RNA + H2O | polyguanylic acid | Hordeum vulgare | 5'-phosphooligonucleotides + 5'-phosphomononucleotides | - |
? | |
| 4.6.1.19 | RNA + H2O | - |
Hordeum vulgare | 3'-phosphomononucleotides | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.2 | 5 | - |
single-stranded DNA | Hordeum vulgare |
| 3.1.30.2 | 5.8 | - |
RNA | Hordeum vulgare |
| 3.1.30.2 | 6.8 | - |
- |
Hordeum vulgare |
| 3.1.30.2 | 7.8 | - |
3'-AMP | Hordeum vulgare |
| 4.6.1.19 | 5.7 | - |
RNAase I | Hordeum vulgare |
| 4.6.1.19 | 6.4 | - |
RNAase II | Hordeum vulgare |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.2 | 5.2 | 7.2 | pH 5.2: about 15% of activity maximum, pH 7.2: about 70% of activity maximum | Hordeum vulgare |
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