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Literature summary extracted from

  • Muller, W.E.G.
    Endoribonuclease IV. A poly(A)-specific ribonuclease from chick oviduct. 1. Purification of the enzyme (1976), Eur. J. Biochem., 70, 241-248.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.6 EDTA presence of 2 mM EDTA results in total inhibition, under 0.2 mM: no effect Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.6 Mg2+ optimum: 1 mM Gallus gallus
3.1.26.6 Mn2+ optimum: 0.5 mM, more effective than Mg2+ Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.6 45000
-
gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.6 RNA + H2O Gallus gallus processing of nuclear RNA to mRNA oligonucleotides average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.6 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.6 15000fold, nearly to homogeneity Gallus gallus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.26.6 endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups forms oligonucleotides with an average chain length of 10 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.6 oviduct
-
Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
3.1.26.6 -20°C, in solution 5, addition of 1 mg/ml albumin, after 6 months 30% of activity were lost Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.6 additional information not: poly(C), poly(U), poly(G), double-stranded poly(A)*poly(U), DNA Gallus gallus ?
-
?
3.1.26.6 poly(A) + H2O
-
Gallus gallus oligonucleotides with an average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups ?
3.1.26.6 RNA + H2O processing of nuclear RNA to mRNA Gallus gallus oligonucleotides average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.6 37
-
-
Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.6 8.7
-
-
Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.26.6 7
-
35% of optimum Gallus gallus
3.1.26.6 9.5
-
40% of optimum Gallus gallus