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Literature summary extracted from

  • Kanaya, S.; Itaya, M.
    Expression, purification, and characterization of a recombinant ribonuclease H from Thermus thermophilus HB8 (1992), J. Biol. Chem., 267, 10184-10192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.4 expression in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 acetonitrile 20%, 50% loss of activity Thermus thermophilus
3.1.26.4 SDS 0.002%, 50% loss of activity Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.4 additional information
-
 additional information
-
 Thermus thermophilus
3.1.26.4 additional information
-
 additional information
-
 Escherichia coli
3.1.26.4 0.0005
-
 M13 DNA/RNA at 37°C Thermus thermophilus
3.1.26.4 0.0011
-
 M13 DNA/RNA at 70°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+
-
 Escherichia coli
3.1.26.4 Mg2+ required Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 21000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Escherichia coli
-
-
-
3.1.26.4 Thermus thermophilus
-
-
-
3.1.26.4 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 M13 DNA/RNA + H2O
-
 Thermus thermophilus ?
-
 ?
3.1.26.4 M13 DNA/RNA + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O
-
 Escherichia coli ribonucleotide 5'-phosphomonoester
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O M13 DNA/RNA hybrid Thermus thermophilus ribonucleotide 5'-phosphomonoester
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O M13 DNA/RNA hybrid Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ribonucleotide 5'-phosphomonoester
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 monomer 1 * 21000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.4 70
-
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.26.4 48.2
-
 melting temperature in presence of 1.2 M guanidinium hydrochloride Escherichia coli
3.1.26.4 55
-
 10 min, 60% loss of activity Escherichia coli
3.1.26.4 82.1
-
 melting temperature in presence of 1.2 M guanidinium hydrochloride Thermus thermophilus
3.1.26.4 90
-
 10 min, stable Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.26.4 additional information
-
 additional information
-
 Thermus thermophilus
3.1.26.4 additional information
-
 additional information
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 8.5 9.5 cleavage of M13 DNA/RNA hybrid Thermus thermophilus