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Literature summary extracted from

  • Sawai, Y.; Uchida, S.; Saito, J.; Sugano, N.; Tsukada, K.
    Two ribonucleases H from cultured plant cells (1979), J. Biochem., 85, 1301-1308.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.4 (NH4)2SO4 30% activation of Mg2+-dependent enzyme Daucus carota
3.1.26.4 sulfhydryl reagent
-
 Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 (NH4)2SO4 200 mM, 80% inhibition of Mn2+-dependent enzyme Daucus carota

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+ Mg2+-dependent enzyme requires 10-15 mM Mg2+ for optimal activity Daucus carota
3.1.26.4 Mn2+ 0.4 mM Mn2+ required for optimal activity, some activity with Mg2+ Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 140000
-
 Mn2+-dependent enzyme, gel filtration Daucus carota
3.1.26.4 180000
-
 Mg2+-dependent enzyme, gel filtration Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Daucus carota
-
-
-
3.1.26.4 Daucus carota
-
 one Mn2+-dependent RNase H and one Mg2+-dependent RNase H
-
3.1.26.4 Daucus carota GD-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 one Mn2+-dependent RNase H and one Mg2+-dependent RNase H Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.4 root
-
 Daucus carota
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 RNA-DNA hybrid + H2O poly(rAdT) Daucus carota ribonucleotide 5'-phosphomonoester a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus ?
3.1.26.4 RNA-DNA hybrid + H2O poly(rAdT) Daucus carota ribonucleotide 5'-phosphomonoester oligonucleotides of various chain length, mainly 3-9 nucleotides in length ?
3.1.26.4 RNA-DNA hybrid + H2O poly(rAdT) Daucus carota GD-2 ribonucleotide 5'-phosphomonoester a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus ?
3.1.26.4 RNA-DNA hybrid + H2O poly(rAdT) Daucus carota GD-2 ribonucleotide 5'-phosphomonoester oligonucleotides of various chain length, mainly 3-9 nucleotides in length ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 8
-
 Mn2+-dependent enzyme Daucus carota
3.1.26.4 9
-
 Mg2+-dependent enzyme Daucus carota