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Literature summary extracted from

  • Kitahara, N.; Sawai, Y.; Tsukada, K.
    Purification and properties of magnesium- and manganese-dependent ribonucleases H from chick embryo (1982), J. Biochem., 92, 855-864.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.4 sulfhydryl reagent required for maximal activity of the Mn2+-dependent enzyme and the Mg2+-dependent enzyme Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 1,10-phenanthroline inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus
3.1.26.4 diphosphate inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus
3.1.26.4 NaF inhibition of Mg2+-dependent enzyme Gallus gallus
3.1.26.4 NEM 2 mM, 80% inhibition of Mn2+-dependent enzyme, complete inhibition of Mg2+-dependent enzyme Gallus gallus
3.1.26.4 PCMB 2 mM, complete inhibition of Mn2+-dependent enzyme and Mg2+-dependent enzyme Gallus gallus
3.1.26.4 spermidine inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus
3.1.26.4 spermine inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+ Mg2+-dependent enzyme requires 15-20 mM Mg2+ for maximal activity Gallus gallus
3.1.26.4 Mg2+ optimal activity with 10-15 mM Gallus gallus
3.1.26.4 Mn2+ 0.4 mM Mn2+ required for optimal activity, some activity with Mg2+ Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 40000
-
Mg2+-dependent enzyme, gel filtration Gallus gallus
3.1.26.4 120000
-
Mn2+-dependent enzyme, gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Gallus gallus
-
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 one Mn2+-dependent RNase H and one Mg2+-dependent RNase H Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.4 embryo
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.4 additional information
-
-
Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 RNA-DNA hybrid + H2O
-
Gallus gallus ribonucleotide 5'-phosphomonoester a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 8.5
-
Mn2+-dependent enzyme Gallus gallus
3.1.26.4 9.5
-
Mg2+-dependent enzyme Gallus gallus