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Literature summary extracted from
- Ribonuclease H from rat liver. I. Partial purification and characterization of nuclear ribonuclease H1 (1978), J. Biochem., 84, 385-393.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.4 | (NH4)2SO4 | optimal concentration: 25 mM | Rattus norvegicus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.4 | DNA | single-stranded or double-stranded, very strong | Rattus norvegicus | |
| 3.1.26.4 | Polyribonucleotides | slight | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.26.4 | additional information | multiple form of ribonuclease H exists in different regions of the rat liver cell | Rattus norvegicus | - |
- |
| 3.1.26.4 | nucleus | nucleoplasm and nucleoli | Rattus norvegicus | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.4 | Mg2+ | required, optimal concentration: 17 mM | Rattus norvegicus | |
| 3.1.26.4 | Mn2+ | optimal concentration 0.6 mM | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 36000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.4 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.4 | partial | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.26.4 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | additional information | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.26.4 | -20°C, 50% v/v glycerol, stable for several months | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.4 | RNA-DNA hybrid + H2O | poly(rUdA) | Rattus norvegicus | ribonucleotide 5'-phosphomonoester | oligoribonucleotides with 3'-hydroxyl and 5'-phosphate termini | ? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 8.5 | 9 | - |
Rattus norvegicus |
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