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Literature summary extracted from

  • Kanno, N.; Sato, M.; Nagahisa, E.; Sato, Y.
    Purification and characterization of tauropine dehydrogenase from the marine sponge Halichondria japonica Kadota (demospongia) (1997), Fish. Sci., 63, 414-420.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.5.1.23 unstable to freezing and thawing Halichondria japonica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.23 pyruvate
-
 Halichondria japonica
1.5.1.23 tauropine substrate inhibition Halichondria japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.23 0.036
-
 NADH
-
 Halichondria japonica
1.5.1.23 0.068
-
 pyruvate
-
 Halichondria japonica
1.5.1.23 0.16
-
 NAD+
-
 Halichondria japonica
1.5.1.23 0.39
-
 tauropine
-
 Halichondria japonica
1.5.1.23 37
-
 taurine
-
 Halichondria japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.23 36500
-
 gel filtration Halichondria japonica
1.5.1.23 37000
-
 1 * 37000, SDS-PAGE Halichondria japonica

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.23 Halichondria japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.23
-
 Halichondria japonica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.23 780
-
-
 Halichondria japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.23 Aminomethane sulfonic acid + pyruvate + NADH at 10.9% of the activity with taurine Halichondria japonica N2-(1-Carboxyethyl)aminomethanesulfonic acid + NAD+ + H2O
-
 ?
1.5.1.23 Gly + pyruvate + NADH at 6.7% of the activity with taurine Halichondria japonica ?
-
 ?
1.5.1.23 Homotaurine + pyruvate + NADH at 8.1% of the activity with taurine Halichondria japonica ?
-
 ?
1.5.1.23 Hypotaurine + pyruvate + NADH at 35.2% of the activity with taurine Halichondria japonica N2-(1-Carboxyethyl)aminoethanesulfinic acid + NAD+ + H2O
-
 ?
1.5.1.23 Taurine + 2-oxobutanoate + NADH ,at 7.9% of the activity with pyruvate Halichondria japonica ?
-
 ?
1.5.1.23 Taurine + 2-oxopentanoate + NADH at 4.8% of the activity with pyruvate Halichondria japonica ?
-
 ?
1.5.1.23 Taurine + 3-hydroxypyruvate + NADH at 4.1% of the activity with pyruvate Halichondria japonica ?
-
 ?
1.5.1.23 Taurine + glyoxylate + NADH at 12.8% of the activity with pyruvate Halichondria japonica ?
-
 ?
1.5.1.23 taurine + oxaloacetate + NADH at 85.8% of the activity with pyruvate Halichondria japonica ?
-
 ?
1.5.1.23 Taurine + pyruvate + NADH r Halichondria japonica Tauropine + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.23 monomer 1 * 37000, SDS-PAGE Halichondria japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.23 6.8
-
 tauropine formation Halichondria japonica
1.5.1.23 9
-
 tauropine oxidation Halichondria japonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.23 5.5 7.4 50% of maximal activity at pH 5.5 and at pH 7.4, tauropine formation Halichondria japonica
1.5.1.23 8 9 pH 8.0: 50% of maximal activity, pH 9.0: optimum, tauropine oxidation Halichondria japonica

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.23 NADH
-
 Halichondria japonica