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Literature summary extracted from

  • Wells, R.D.; Klein, R.D.; Singleton, C.K.
    Type II restriction enzymes (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 157-191.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.21.4 0.0004
-
 ColE1 DNA EcoRI, activated by Co2+, 37°C Escherichia coli
3.1.21.4 0.003 0.008 ColE1 DNA EcoRI, activated by Mg2+ Escherichia coli
3.1.21.4 0.005
-
 pBR322 DNA EcoRI, 37°C Escherichia coli
3.1.21.4 0.01
-
 Lambda DNA EcoRI, 37°C Escherichia coli
3.1.21.4 0.03
-
 d(G-G-T-5-bromodeoxyuridine-A-A-C-C)
-
 Haemophilus parainfluenzae
3.1.21.4 0.03
-
 SV40 DNA EcoRI, 37°C Escherichia coli
3.1.21.4 0.03
-
 d(G-G-T-5-bromodeoxyuridine-A-A-C-C) Hpa, 25°C Bacillus amyloliquefaciens
3.1.21.4 0.18
-
 d(G-G-T-T-A-A-C-C) HpaI, 25°C Haemophilus parainfluenzae
3.1.21.4 0.3 0.36 pJC80 DNA BamHI, 37°C Bacillus amyloliquefaciens
3.1.21.4 0.9
-
 pJC linearized plasmid DNA BamHI, 37°C Bacillus amyloliquefaciens
3.1.21.4 7
-
 d(pT-G-A-A-T-T-C-A) EcoR1, 12 C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.4 Co2+ can replace for Mg2+ Escherichia coli
3.1.21.4 Mg2+ required Staphylococcus aureus
3.1.21.4 Mg2+ required Haemophilus influenzae
3.1.21.4 Mg2+ required Bacillus subtilis
3.1.21.4 Mg2+ required Thermus thermophilus
3.1.21.4 Mg2+ required Escherichia coli
3.1.21.4 Mg2+ required Geobacillus stearothermophilus
3.1.21.4 Mg2+ required Cereibacter sphaeroides
3.1.21.4 Mg2+ required Lysinibacillus sphaericus
3.1.21.4 Mg2+ required Serratia marcescens
3.1.21.4 Mg2+ required Enterococcus faecalis
3.1.21.4 Mg2+ required Streptococcus pneumoniae
3.1.21.4 Mg2+ required Bacillus amyloliquefaciens
3.1.21.4 Mg2+ required Streptomyces albus
3.1.21.4 Mg2+ required [Bacillus] caldolyticus
3.1.21.4 Mg2+ required Fusobacterium nucleatum
3.1.21.4 Mg2+ required Avibacterium paragallinarum
3.1.21.4 Mg2+ required Desulfovibrio desulfuricans
3.1.21.4 Mg2+ required Trichormus variabilis
3.1.21.4 Mg2+ required Haemophilus aegyptius
3.1.21.4 Mg2+ required Haemophilus parahaemolyticus
3.1.21.4 Mg2+ required Xanthomonas citri pv. malvacearum
3.1.21.4 Mg2+ required Providencia stuartii
3.1.21.4 Mg2+ required Cellulosimicrobium cellulans
3.1.21.4 Mg2+ required Xanthomonas vasicola
3.1.21.4 Mg2+ required Xanthomonas campestris pv. badrii
3.1.21.4 Mg2+ required Herpetosiphon aurantiacus
3.1.21.4 Mg2+ required Haemophilus haemolyticus
3.1.21.4 Mg2+ required Haemophilus parainfluenzae
3.1.21.4 Mg2+ required Moraxella bovis
3.1.21.4 Mg2+ optimal concentration is 20 mM Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.4 20000
-
 enzyme DpnI Streptococcus pneumoniae
3.1.21.4 22000
-
 enzyme BamHI Bacillus amyloliquefaciens
3.1.21.4 24000
-
 enzyme HhaII Haemophilus haemolyticus
3.1.21.4 25000
-
 enzyme BclI [Bacillus] caldolyticus
3.1.21.4 26000
-
 enzyme BstI Geobacillus stearothermophilus
3.1.21.4 27000
-
 enzyme BglII Bacillus subtilis
3.1.21.4 28500
-
 enzyme EcoRI Escherichia coli
3.1.21.4 31000
-
 enzyme BglI Bacillus subtilis
3.1.21.4 40000
-
 enzyme HpaII Haemophilus parainfluenzae
3.1.21.4 68000
-
 enzyme BsuI Bacillus subtilis
3.1.21.4 70000
-
 enzyme HindII Haemophilus influenzae
3.1.21.4 70000
-
 enzyme DpnII Streptococcus pneumoniae
3.1.21.4 95000
-
 enzyme Tth111II Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.4 Avibacterium paragallinarum
-
-
-
3.1.21.4 Bacillus amyloliquefaciens
-
-
-
3.1.21.4 Bacillus subtilis
-
-
-
3.1.21.4 Cellulosimicrobium cellulans
-
-
-
3.1.21.4 Cereibacter sphaeroides
-
-
-
3.1.21.4 Desulfovibrio desulfuricans
-
-
-
3.1.21.4 Enterococcus faecalis
-
-
-
3.1.21.4 Escherichia coli
-
-
-
3.1.21.4 Fusobacterium nucleatum
-
-
-
3.1.21.4 Geobacillus stearothermophilus
-
-
-
3.1.21.4 Haemophilus aegyptius
-
-
-
3.1.21.4 Haemophilus haemolyticus
-
 enzyme HhaII
-
3.1.21.4 Haemophilus influenzae
-
-
-
3.1.21.4 Haemophilus parahaemolyticus
-
 enzyme HphI
-
3.1.21.4 Haemophilus parainfluenzae
-
 enzyme HpaII
-
3.1.21.4 Haemophilus parainfluenzae
-
 enzymes HpaI
-
3.1.21.4 Herpetosiphon aurantiacus
-
-
-
3.1.21.4 Lysinibacillus sphaericus
-
-
-
3.1.21.4 Moraxella bovis
-
 enzymes MboI and MboII
-
3.1.21.4 Providencia stuartii
-
-
-
3.1.21.4 Serratia marcescens
-
-
-
3.1.21.4 Staphylococcus aureus
-
 Sau96I
-
3.1.21.4 Staphylococcus aureus Sau96I
-
 Sau96I
-
3.1.21.4 Streptococcus pneumoniae
-
 enzyme Dpn II
-
3.1.21.4 Streptococcus pneumoniae
-
 enzyme DpnI
-
3.1.21.4 Streptomyces achromogenes
-
-
-
3.1.21.4 Streptomyces albus
-
-
-
3.1.21.4 Thermus thermophilus
-
-
-
3.1.21.4 Trichormus variabilis
-
-
-
3.1.21.4 Xanthomonas campestris pv. badrii
-
 enzyme XbaI
-
3.1.21.4 Xanthomonas citri pv. malvacearum
-
-
-
3.1.21.4 Xanthomonas vasicola
-
-
-
3.1.21.4 [Bacillus] caldolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.4 ColE1 DNA + H2O
-
 Escherichia coli ?
-
 ?
3.1.21.4 d(G-G-T-5-bromodeoxyuridine-A-A-C-C) + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.1.21.4 d(G-G-T-5-bromodeoxyuridine-A-A-C-C) + H2O
-
 Haemophilus parainfluenzae ?
-
 ?
3.1.21.4 d(G-G-T-T-5'-bromodeoxyuridine-C-C) + H2O
-
 Haemophilus parainfluenzae ?
-
 ?
3.1.21.4 d(G-G-T-T-A-A-C-C) + H2O
-
 Haemophilus parainfluenzae ?
-
 ?
3.1.21.4 d(pT-G-A-A-T-T-C-A) + H2O
-
 Escherichia coli ?
-
 ?
3.1.21.4 DNA + H2O
-
 Staphylococcus aureus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Haemophilus influenzae double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Bacillus subtilis double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Thermus thermophilus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Geobacillus stearothermophilus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Cereibacter sphaeroides double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Lysinibacillus sphaericus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Serratia marcescens double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Enterococcus faecalis double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Streptococcus pneumoniae double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Bacillus amyloliquefaciens double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Streptomyces albus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Streptomyces achromogenes double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 [Bacillus] caldolyticus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Fusobacterium nucleatum double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Avibacterium paragallinarum double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Desulfovibrio desulfuricans double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Trichormus variabilis double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Haemophilus aegyptius double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Haemophilus parahaemolyticus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Xanthomonas citri pv. malvacearum double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Providencia stuartii double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Cellulosimicrobium cellulans double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Xanthomonas vasicola double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Xanthomonas campestris pv. badrii double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Herpetosiphon aurantiacus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Haemophilus haemolyticus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Haemophilus parainfluenzae double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Moraxella bovis double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O ColE1 DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O pBR322 DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O SV40 DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O lambda DNA Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 DNA + H2O
-
 Staphylococcus aureus Sau96I double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 lambda DNA + H2O
-
 Escherichia coli ?
-
 ?
3.1.21.4 NTP14 DNA + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.1.21.4 pBR322 DNA + H2O
-
 Escherichia coli ?
-
 ?
3.1.21.4 pJC linearized plasmid DNA + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.1.21.4 pJC80 DNA + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.1.21.4 SV40 DNA + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.4 AluI
-
 Cellulosimicrobium cellulans
3.1.21.4 BglI
-
 Bacillus subtilis
3.1.21.4 BglII
-
 Bacillus subtilis
3.1.21.4 DpnI
-
 Streptococcus pneumoniae
3.1.21.4 DpnII
-
 Streptococcus pneumoniae
3.1.21.4 HgiAI
-
 Herpetosiphon aurantiacus
3.1.21.4 HindII
-
 Haemophilus influenzae
3.1.21.4 HindIII
-
 Haemophilus influenzae
3.1.21.4 HinfI
-
 Haemophilus influenzae
3.1.21.4 PstI
-
 Providencia stuartii
3.1.21.4 RsaI
-
 Cereibacter sphaeroides
3.1.21.4 sau96I
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.4 25
-
 XmaIII Xanthomonas citri pv. malvacearum
3.1.21.4 25 37 SmaI Serratia marcescens
3.1.21.4 30
-
 Sau96I and Sau3AI Staphylococcus aureus
3.1.21.4 30 40 BglI and BglII Bacillus subtilis
3.1.21.4 30 45 HgiAI Herpetosiphon aurantiacus
3.1.21.4 34
-
 RsaI Cereibacter sphaeroides
3.1.21.4 37
-
 HaeII Haemophilus aegyptius
3.1.21.4 37
-
 XhoI Xanthomonas vasicola
3.1.21.4 37
-
 SalI and SalPI Streptomyces albus
3.1.21.4 37
-
 DdeI Desulfovibrio desulfuricans
3.1.21.4 37
-
 MboI and MboII Moraxella bovis
3.1.21.4 37
-
 Sfa Enterococcus faecalis
3.1.21.4 37
-
 FnuEI, FnuHI and FnuDII Fusobacterium nucleatum
3.1.21.4 37
-
 HpaII Haemophilus parainfluenzae
3.1.21.4 37
-
 XmaI and XmaII Xanthomonas citri pv. malvacearum
3.1.21.4 37
-
 HphI Haemophilus parahaemolyticus
3.1.21.4 37
-
 XbaI Xanthomonas campestris pv. badrii
3.1.21.4 37
-
 HgaI Avibacterium paragallinarum
3.1.21.4 37
-
 HinfI, Hind II and HindIII Haemophilus influenzae
3.1.21.4 37
-
 HhaI and HhaII Haemophilus haemolyticus
3.1.21.4 37
-
 DpnI, DPNII Streptococcus pneumoniae
3.1.21.4 37
-
 Ava I and AvaII Trichormus variabilis
3.1.21.4 37
-
 AluI Cellulosimicrobium cellulans
3.1.21.4 37
-
 PstI Providencia stuartii
3.1.21.4 37
-
 Bsp Lysinibacillus sphaericus
3.1.21.4 37
-
 EcoRI Escherichia coli
3.1.21.4 37
-
 BamHI Bacillus amyloliquefaciens
3.1.21.4 45
-
 HpaI Haemophilus parainfluenzae
3.1.21.4 50
-
 BclI [Bacillus] caldolyticus
3.1.21.4 55
-
 BstPI Streptomyces achromogenes
3.1.21.4 60 65 BstI Geobacillus stearothermophilus
3.1.21.4 60 70 Tth111I Thermus thermophilus
3.1.21.4 65 70 Tth111II Thermus thermophilus
3.1.21.4 70
-
 HaeIII Haemophilus aegyptius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.21.4 0.0167
-
 ColE1 DNA EcoRI, activated by Co2+, 37°C Escherichia coli
3.1.21.4 0.0217
-
 Lambda DNA EcoR1, 37°C Escherichia coli
3.1.21.4 0.025
-
 SV40 DNA EcoR1, 37°C Escherichia coli
3.1.21.4 0.0267
-
 NTP14 DNA BamHI, 37°C Bacillus amyloliquefaciens
3.1.21.4 0.03
-
 pBR322 DNA EcoRI, pBR322, 37°C Escherichia coli
3.1.21.4 0.0367
-
 pJC80 DNA BamHI, 37°C Bacillus amyloliquefaciens
3.1.21.4 0.0667
-
 d(pT-G-A-A-T-T-C-A) EcoRI, 12°C Escherichia coli
3.1.21.4 0.0667 0.133 ColE1 DNA EcoRI, activated by Mg2+ Escherichia coli
3.1.21.4 600
-
 d(G-G-T-5-bromodeoxyuridine-A-A-C-C)
-
 Haemophilus parainfluenzae
3.1.21.4 600
-
 d(G-G-T-5-bromodeoxyuridine-A-A-C-C) Hpa, 25°C Bacillus amyloliquefaciens
3.1.21.4 2830
-
 d(G-G-T-T-A-A-C-C) HpaI, 25°C Haemophilus parainfluenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.4 6.8
-
 HpaII Haemophilus parainfluenzae
3.1.21.4 7.1 7.5 EcoRI Escherichia coli
3.1.21.4 7.4
-
 PstI Providencia stuartii
3.1.21.4 7.4
-
 Sau96I Staphylococcus aureus
3.1.21.4 7.4
-
 HphI Haemophilus parahaemolyticus
3.1.21.4 7.4
-
 Tth111I Thermus thermophilus
3.1.21.4 7.4
-
 HaeII Haemophilus aegyptius
3.1.21.4 7.4
-
 HhaI Haemophilus haemolyticus
3.1.21.4 7.4
-
 BclI [Bacillus] caldolyticus
3.1.21.4 7.5 9 AvaII Trichormus variabilis
3.1.21.4 7.5
-
 HinfI Haemophilus influenzae
3.1.21.4 7.5
-
 Tth111II Thermus thermophilus
3.1.21.4 7.5
-
 HaeIII Haemophilus aegyptius
3.1.21.4 7.5
-
 Sau3AI Staphylococcus aureus
3.1.21.4 7.5
-
 XmaI Xanthomonas citri pv. malvacearum
3.1.21.4 7.5
-
 Sfa Enterococcus faecalis
3.1.21.4 7.5
-
 SmaI Serratia marcescens
3.1.21.4 7.5
-
 DdeI Desulfovibrio desulfuricans
3.1.21.4 7.5 8.5 HgiAI Herpetosiphon aurantiacus
3.1.21.4 7.5 8 HindII Haemophilus influenzae
3.1.21.4 7.5 8 BstI Geobacillus stearothermophilus
3.1.21.4 7.5 8.8 AvaI Trichormus variabilis
3.1.21.4 7.6
-
 DpnI and DpnII Streptococcus pneumoniae
3.1.21.4 7.7 8.1 HpaI Haemophilus parainfluenzae
3.1.21.4 7.9
-
 AluI Cellulosimicrobium cellulans
3.1.21.4 7.9
-
 MboI and MboII Moraxella bovis
3.1.21.4 7.9
-
 SalI and SalPI Streptomyces albus
3.1.21.4 7.9
-
 XmaII Xanthomonas citri pv. malvacearum
3.1.21.4 7.9
-
 HgaI Avibacterium paragallinarum
3.1.21.4 7.9
-
 RsaI Cereibacter sphaeroides
3.1.21.4 7.9
-
 Fnu4HI, FnuDII and FnuEI Fusobacterium nucleatum
3.1.21.4 7.9
-
 XhoI Xanthomonas vasicola
3.1.21.4 7.9
-
 XbaI Xanthomonas campestris pv. badrii
3.1.21.4 8 8.5 Bsp Lysinibacillus sphaericus
3.1.21.4 8 9 HindIII Haemophilus influenzae
3.1.21.4 8.2
-
-
 Lysinibacillus sphaericus
3.1.21.4 8.2
-
 XmaIII Xanthomonas citri pv. malvacearum
3.1.21.4 8.5
-
 BamHI Bacillus amyloliquefaciens
3.1.21.4 9
-
 HhaII Haemophilus haemolyticus
3.1.21.4 9.5
-
 BglI and BglII Bacillus subtilis