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Literature summary extracted from

  • Funakoshi, A.; Tsubota, Y.; Wakasugi, H.; Ibayashi, H.; Takagi, Y.
    Purification and properties of human pancreatic deoxyribonuclease I (1977), J. Biochem., 82, 1771-1777.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 Ca2+ 80% inhibition at 0.1 mM Homo sapiens
3.1.21.1 EGTA activity inhibited Homo sapiens
3.1.21.1 G-actin complete inhibition at 0.001 mg Homo sapiens
3.1.21.1 iodoacetate complete inhibition Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.21.1 extracellular secreted into duodenal juice Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ activation synergistic with Mg2+ Homo sapiens
3.1.21.1 Mg2+ optimal concentration 5 mM Homo sapiens
3.1.21.1 Mg2+ activation synergistic with Ca2+ Homo sapiens
3.1.21.1 Mn2+ 120% activity of Mg2+ activated enzyme at 10 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 30000
-
 gel filtration, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Homo sapiens hydrolysis of both double- and single-stranded DNA 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 near homogeneity by fractionation steps Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 duodenal juice
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 0.033
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.21.1 -20°C, 10 mM Tris-HCl, pH 7.5, 5 mM CaCl2, 30% polyethylene glycol, more than 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O hydrolysis of both double- and single-stranded DNA Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 heat-denatured DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 supercoiled plasmid DNA + H2O
-
 Homo sapiens linear DNA + ?
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.21.1 60
-
 rather stable up to Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1
-
-
 stable for 7 days Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 7.2 7.6 in 33 mM Tris-HCl buffer Homo sapiens