Literature summary extracted from
Moore, S.
Pancreatic DNase (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 281-296.
No PubMed abstract available
Application
EC Number |
Application |
Comment |
Organism |
---|
3.1.21.1 |
analysis |
study of chromatin structure, visualizing cellular microfilaments by DNase I-actin interaction |
Bos taurus |
3.1.21.1 |
medicine |
inhibition of viral synthesis by DNase I in cell cultures |
Bos taurus |
General Stability
EC Number |
General Stability |
Organism |
---|
3.1.21.1 |
extremely sensitive to inactivation by proteases in the absence of Ca2+ |
Bos taurus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.21.1 |
2-Nitro-5-thiocyanobenzoic acid |
inactivation by cleavage of peptide chain at positions 14, 40, 72 and 135 |
Bos taurus |
|
3.1.21.1 |
Ca2+ |
inhibitory above 1 mM |
Bos taurus |
|
3.1.21.1 |
carbodiimide |
presence of divalent cations slows the rate of inactivation |
Bos taurus |
|
3.1.21.1 |
EGTA |
inhibition at 0.01 mM in the presence of 2.5 mM Mg2+ |
Bos taurus |
|
3.1.21.1 |
G-actin |
DNase I causes depolymerization of F-actin to form a stable complex of 1 mol DNase I with 1 mol G-actin, this complex inhibits DNase I activity |
Bos taurus |
|
3.1.21.1 |
iodoacetate |
inhibition in the presence of Mn2+ or Ca2+ at pH 7.2 |
Bos taurus |
|
3.1.21.1 |
methanesulfonylchloride |
inactivation at pH 5.0 |
Bos taurus |
|
3.1.21.1 |
N-bromosuccinimide |
inactivation by modification at Trp155 |
Bos taurus |
|
3.1.21.1 |
NaCl |
Na-DNA is inhibitory |
Bos taurus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.21.1 |
Ba2+ |
can substitute for Ca2+, but less effective |
Bos taurus |
|
3.1.21.1 |
Ca2+ |
resistance to proteolysis conveyed by Ca2+ |
Bos taurus |
|
3.1.21.1 |
Ca2+ |
activation synergistic with Mg2+ |
Bos taurus |
|
3.1.21.1 |
Co2+ |
can substitute for Mg2+, but only 10% efficiency |
Bos taurus |
|
3.1.21.1 |
Mg2+ |
enzyme about 99.5% inactive if Ca2+ contamination is reduced to a minimum, must bind to the substrate, whereas Ca2+ must bind to the enzyme |
Bos taurus |
|
3.1.21.1 |
Mn2+ |
can substitute for Mg2+ |
Bos taurus |
|
3.1.21.1 |
Sr2+ |
can substitute for Ca2+, but less effective |
Bos taurus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.21.1 |
30070 |
- |
calculated from amino acid and carbohydrate composition for DNase A |
Bos taurus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.21.1 |
double-stranded DNA + H2O |
Bos taurus |
double-strand scission obtained if affinity for the substrate is at a maximum in the presence of both Ca2+ and Mg2+, single-strand scission and changes in specificity are associated with suboptimal concentrations of Ca2+ |
5'-phosphooligonucleotides + ? |
in the presence of divalent cations that give less maximum activity consistent yields of long oligonucleotides lacking dA at the 3'-end |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.21.1 |
Bos taurus |
- |
4 active compounds: A, B, C, D |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.1.21.1 |
glycoprotein |
DNase D contains sialic acid in the carbohydrate portion |
Bos taurus |
3.1.21.1 |
glycoprotein |
carbohydrate-chain at Asn 18 |
Bos taurus |
3.1.21.1 |
glycoprotein |
DNase A with a neutral carbohydrate side-chain, DNase B with a sialic acid carbohydrate side-chain |
Bos taurus |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.21.1 |
conconavalin A-agarose as an effective step for DNases with neutral carbohydrate side-chains, preparing a protease free DNase I |
Bos taurus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
3.1.21.1 |
37°C, protease free preparation, absence of Ca2+, pH 8.0, more than 10 days |
Bos taurus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.21.1 |
crab d(A-T) polymer + H2O |
unique polymer of alterating A and T contains about 3% G and C residues integrated into its structure |
Bos taurus |
5'-hexanucleotides + ? |
enriched in C and G, sugar specificity may be limited to the nucleotide following the point of cleavage |
? |
|
3.1.21.1 |
double-stranded DNA + H2O |
double-strand scission obtained if affinity for the substrate is at a maximum in the presence of both Ca2+ and Mg2+, single-strand scission and changes in specificity are associated with suboptimal concentrations of Ca2+ |
Bos taurus |
5'-phosphooligonucleotides + ? |
in the presence of divalent cations that give less maximum activity consistent yields of long oligonucleotides lacking dA at the 3'-end |
? |
|
3.1.21.1 |
NO2-Ph-pdTp-NO2Ph + H2O |
rapidly hydrolyzed at a single bond |
Bos taurus |
p-nitrophenol + NO2-Ph-pdT-3'-phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.21.1 |
DNase A |
- |
Bos taurus |
3.1.21.1 |
DNase B |
- |
Bos taurus |
3.1.21.1 |
DNase C |
- |
Bos taurus |
3.1.21.1 |
DNase D |
- |
Bos taurus |