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Literature summary extracted from

  • Kreuder, V.; Dieckhoff, J.; Sittig, M.; Mannherz, H.G.
    Isolation, characterization and crystallization of deoxyribonuclease I from bovine and rat parotid gland and its characterization with rabbit skeletal muscle actin (1984), Eur. J. Biochem., 139, 389-400.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 2-Nitro-5-thiocyanobenzoic acid inhibition at identical rates Bos taurus
3.1.21.1 2-Nitro-5-thiocyanobenzoic acid inhibition at identical rates Rattus sp.
3.1.21.1 EDTA
-
 Bos taurus
3.1.21.1 EDTA
-
 Rattus sp.
3.1.21.1 G-actin
-
 Bos taurus
3.1.21.1 G-actin
-
 Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.21.1 additional information
-
 Double-stranded DNA 20 microgram/ml Bos taurus
3.1.21.1 additional information
-
 Double-stranded DNA 25 microgram/ml Rattus sp.
3.1.21.1 additional information
-
 Double-stranded DNA values in microgram DNA/ml Bos taurus
3.1.21.1 additional information
-
 Double-stranded DNA values in microgram DNA/ml Rattus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ activation at 0.1 mM Bos taurus
3.1.21.1 Ca2+ activation at 0.1 mM Rattus sp.
3.1.21.1 Ca2+ activation synergistic with Mg2+ Bos taurus
3.1.21.1 Ca2+ activation synergistic with Mg2+ Rattus sp.
3.1.21.1 Mg2+ 5% activation Bos taurus
3.1.21.1 Mg2+ 5% activation Rattus sp.
3.1.21.1 Mg2+ activation synergistic with Ca2+ Bos taurus
3.1.21.1 Mg2+ activation synergistic with Ca2+ Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 32000
-
 SDS-PAGE Rattus sp.
3.1.21.1 34000
-
 SDS-PAGE Bos taurus
3.1.21.1 34000
-
 SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Bos taurus preference for double-stranded DNA 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O Rattus sp. preference for double-stranded DNA 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Bos taurus
-
-
-
3.1.21.1 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.21.1 glycoprotein
-
 Rattus sp.
3.1.21.1 glycoprotein DNase A with a neutral carbohydrate side-chain, DNase B with a sialic acid carbohydrate side-chain Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 high homogeneity by chromatography steps Bos taurus
3.1.21.1 high homogeneity by chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 parotid gland
-
 Bos taurus
-
3.1.21.1 parotid gland
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
 units defined as absorbance at 260 nm/ml assay solution Bos taurus
3.1.21.1 additional information
-
 units defined as absorbance at 260 nm/ml assay solution Rattus sp.
3.1.21.1 additional information
-
 222 units/mg protein Rattus sp.
3.1.21.1 additional information
-
 938 units/mg protein Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 DNA + H2O
-
 Rattus sp. 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 DNA + H2O no preference for any nucleotide Bos taurus 5'-phosphotrinucleotides + ? as the main products ?
3.1.21.1 DNA + H2O no preference for any nucleotide Rattus sp. 5'-phosphotrinucleotides + ? as the main products ?
3.1.21.1 double-stranded DNA + H2O preference for double-stranded DNA Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O preference for double-stranded DNA Rattus sp. 5'-phosphooligonucleotides + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 7.8
-
-
 Bos taurus
3.1.21.1 7.8
-
-
 Rattus sp.
3.1.21.1 8.2
-
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.21.1 4
-
 activity completely lost Bos taurus
3.1.21.1 4
-
 activity completely lost Rattus sp.