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Literature summary extracted from

  • Ito, K.; Minamiura, N.; Yamamato, T.
    Human urine DNase I: Immunological identity with human pancreatic DNase I, and enzyme and proteochemical properties of the enzyme (1984), J. Biochem., 95, 1399-1406.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.21.1 less active in phosphate buffer Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.21.1 extracellular secreted into urine Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+
-
 Homo sapiens
3.1.21.1 Co2+
-
 Homo sapiens
3.1.21.1 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 41000
-
 SDS-PAGE, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Homo sapiens
-
 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.21.1 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 to homogeneity by 6step chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
 units defined as absorbance at 260 nm/ml assay solution Homo sapiens
3.1.21.1 additional information
-
 1683 units/mg protein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O degrades native DNA 2times faster than denatured DNA Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 heat-denatured DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 poly(dA) + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 poly(dT) + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1 45
-
 stable up to at pH 6.5, loss of activity at 60°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 6.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.1 5 9
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.21.1 5 9 stable within the range for 24 h at 4°C Homo sapiens