EC Number | General Stability | Organism |
---|---|---|
3.1.21.1 | sensitives to repeated freezing and thawing | Coprinopsis cinerea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | EGTA | activity inhibited | Coprinopsis cinerea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | Ca2+ | in presence of Ca2+ less sensitive to buffer strength | Coprinopsis cinerea | |
3.1.21.1 | Mg2+ | absolute requirement for activity, highest activity if both Mg2+ and Ca2+ are present | Coprinopsis cinerea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 12000 | - |
SDS-PAGE | Coprinopsis cinerea |
3.1.21.1 | 14000 | - |
SDS-PAGE | Coprinopsis cinerea |
3.1.21.1 | 22000 | - |
gel filtration | Coprinopsis cinerea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.1 | double-stranded DNA + H2O | Coprinopsis cinerea | preference for single-stranded and supercoiled DNA | 5'-phosphooligonucleotides + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.1 | Coprinopsis cinerea | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.21.1 | basidiocarp | - |
Coprinopsis cinerea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | additional information | - |
21 units/min/ng DNA digested | Coprinopsis cinerea |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.21.1 | -70°C, 10 mM Tris/HCl, pH 7.4, 50% v/v glycerol, 2 years | Coprinopsis cinerea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.1 | double-stranded DNA + H2O | preference for single-stranded and supercoiled DNA | Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
3.1.21.1 | relaxed circular plasmid DNA + H2O | - |
Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
3.1.21.1 | single-stranded circular DNA + H2O | preference for single-stranded regions | Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
3.1.21.1 | supercoiled plasmid DNA + H2O | - |
Coprinopsis cinerea | linear DNA + ? | formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.21.1 | dimer | 1 * 12000 and 1 * 14000, gel filtration, SDS-PAGE | Coprinopsis cinerea |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 37 | - |
- |
Coprinopsis cinerea |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 37 | 60 | activity remains unchanged | Coprinopsis cinerea |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 60 | - |
stable for 20 min | Coprinopsis cinerea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 7 | - |
- |
Coprinopsis cinerea |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 5 | 8 | 64% activity below pH 5.0, 80% activity above pH 8.0 | Coprinopsis cinerea |