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Literature summary extracted from

  • Yasuda, T.; Awazu, S.; Sato, W.; Iida, R.; Tanaka, Y.; Kishi, K.
    Human gentically polymorphic deoxyribonuclease: purification, characterization, and multiplicity of urine deoxyribonuclease I (1990), J. Biochem., 108, 393-398.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 EDTA
-
 Homo sapiens
3.1.21.1 EGTA inhibition at 1 mM Homo sapiens
3.1.21.1 G-actin 50% inhibition at 0.002 mg/ml Homo sapiens
3.1.21.1 NaCl decreasing activity with increasing ionic strength Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.21.1 extracellular secreted into urine Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+
-
 Homo sapiens
3.1.21.1 Co2+ activation at 10 mM Homo sapiens
3.1.21.1 Mg2+ activation at 10 mM, 2fold activation, if 1 mM CaCl2 is added Homo sapiens
3.1.21.1 Mn2+ activation at 10 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 34000
-
 SDS-PAGE, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Homo sapiens
-
 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.21.1 glycoprotein contains fucose, mannose, galactose, glucosamine, sialic acid Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 to homogeneity by 6step chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
 units defined as absorbance at 260 nm/ml assay solution Homo sapiens
3.1.21.1 additional information
-
 1683 units/mg protein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O degrades native DNA 2times faster than denatured DNA Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 heat-denatured DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 monomer 1 * 34000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1 45
-
 stable up to, more stable up to 60°C, if Ca2+ is present Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 6.5
-
 in the presence of 10 mM MgCl2 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.1 4.5 9.5
-
 Homo sapiens