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Literature summary extracted from
- Glyoxalase I and glyoxalase II from Aloe vera: purification, characterization and comparison with animal glyoxalases (1990), Biochem. Int., 22, 411-418.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.2.6 | N,S-(dicarbobenzoxy)glutathione | - |
Aloe vera |  |
| 3.1.2.6 | octyl-S-glutathione | - |
Aloe vera | |
| 3.1.2.6 | S-benzyloxycarbonylglutathione | - |
Aloe vera | |
| 3.1.2.6 | S-fluorenylmethyloxycarbonylglutathione | - |
Aloe vera | |
| 4.4.1.5 | EDTA | 1 mM | Aloe vera | |
| 4.4.1.5 | octyl-S-glutathione | - |
Aloe vera | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.6 | 0.15 | - |
(S)-D-lactoylglutathione | - |
Aloe vera | |
| 3.1.2.6 | 0.2 | - |
S-acetylglutathione | - |
Aloe vera | |
| 4.4.1.5 | 1.4 | - |
glutathione-methylglyoxal hemithioacetal | - |
Aloe vera | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.6 | 27000 | - |
gel filtration | Aloe vera |
| 4.4.1.5 | 44000 | - |
gel filtration | Aloe vera |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.6 | Aloe vera | - |
- |
- |
| 4.4.1.5 | Aloe vera | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.6 | - |
Aloe vera |
| 4.4.1.5 | - |
Aloe vera |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.2.6 | rind | outer green rind | Aloe vera | - |
| 4.4.1.5 | rind | green | Aloe vera | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.6 | 536.4 | - |
- |
Aloe vera |
| 4.4.1.5 | 200 | - |
- |
Aloe vera |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.6 | (S)-D-lactoylglutathione + H2O | - |
Aloe vera | D-lactate + glutathione | - |
? | |
| 3.1.2.6 | S-acetylglutathione + H2O | - |
Aloe vera | acetate + glutathione | - |
? | |
| 4.4.1.5 | glutathione + methylglyoxal | - |
Aloe vera | S-lactoylglutathione | - |
? | |
| 4.4.1.5 | glutathione-methylglyoxal hemithioacetal | - |
Aloe vera | (R)-S-lactoylglutathione | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.6 | monomer | 1 * 27000, SDS-PAGE | Aloe vera |
| 4.4.1.5 | monomer | 1 * 44000, SDS-PAGE | Aloe vera |
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