BRENDA - Enzyme Database

Demonstration of glyoxalase II in rat liver mitochondria. Partial purification and occurrence in multiple forms

Talesa, V.; Uotila, L.; Koivusalo, M.; Principato, G.; Giovannini, E.; Rosi, G.; Biochim. Biophys. Acta 955, 103-110 (1988)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.6
0.044
-
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.05
-
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.16
-
S-succinylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.213
-
S-formylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.214
-
S-propionylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.242
-
S-propionylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.325
-
S-succinylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.378
-
S-propionylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.433
-
S-D-lactoylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.44
-
S-D-lactoylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.568
-
S-acetylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.662
-
S-acetylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.703
-
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.841
-
S-Acetoacetylglutathione
cytosolic enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.6
cytosol
-
Rattus norvegicus
5829
-
3.1.2.6
mitochondrion
10-15% of the total glyoxalase II activity in liver. 56% of the activity resides in the matrix, 19% in the intermembrane space. 3 isoforms are exclusively mitochondrial
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.6
21000
-
-
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.6
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.6
partial, multiple forms
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.6
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.6
112
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.6
lactoylglutathione + H2O
-
134177
Rattus norvegicus
D-lactate + glutathione
-
-
-
?
3.1.2.6
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione + H2O
-
134177
Rattus norvegicus
beta-ethoxy-alpha-D-hydroxybutyrate + glutathione
-
-
-
?
3.1.2.6
S-acetoacetylglutathione + H2O
-
134177
Rattus norvegicus
acetoacetate + glutathione
-
-
-
?
3.1.2.6
S-acetylglutathione + H2O
-
134177
Rattus norvegicus
acetate + glutathione
-
-
-
?
3.1.2.6
S-formylglutathione + H2O
-
134177
Rattus norvegicus
formate + glutathione
-
-
-
?
3.1.2.6
S-propionylglutathione + H2O
-
134177
Rattus norvegicus
propionate + glutathione
-
-
-
?
3.1.2.6
S-succinylglutathione + H2O
-
134177
Rattus norvegicus
succinate + glutathione
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.6
0.044
-
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.05
-
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.16
-
S-succinylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.213
-
S-formylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.214
-
S-propionylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.242
-
S-propionylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.325
-
S-succinylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.378
-
S-propionylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.433
-
S-D-lactoylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.44
-
S-D-lactoylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.568
-
S-acetylglutathione
cytosolic enzyme
Rattus norvegicus
3.1.2.6
0.662
-
S-acetylglutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.703
-
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
mitochondrial enzyme
Rattus norvegicus
3.1.2.6
0.841
-
S-Acetoacetylglutathione
cytosolic enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.6
cytosol
-
Rattus norvegicus
5829
-
3.1.2.6
mitochondrion
10-15% of the total glyoxalase II activity in liver. 56% of the activity resides in the matrix, 19% in the intermembrane space. 3 isoforms are exclusively mitochondrial
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.6
21000
-
-
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.6
partial, multiple forms
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.6
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.6
112
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.6
lactoylglutathione + H2O
-
134177
Rattus norvegicus
D-lactate + glutathione
-
-
-
?
3.1.2.6
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione + H2O
-
134177
Rattus norvegicus
beta-ethoxy-alpha-D-hydroxybutyrate + glutathione
-
-
-
?
3.1.2.6
S-acetoacetylglutathione + H2O
-
134177
Rattus norvegicus
acetoacetate + glutathione
-
-
-
?
3.1.2.6
S-acetylglutathione + H2O
-
134177
Rattus norvegicus
acetate + glutathione
-
-
-
?
3.1.2.6
S-formylglutathione + H2O
-
134177
Rattus norvegicus
formate + glutathione
-
-
-
?
3.1.2.6
S-propionylglutathione + H2O
-
134177
Rattus norvegicus
propionate + glutathione
-
-
-
?
3.1.2.6
S-succinylglutathione + H2O
-
134177
Rattus norvegicus
succinate + glutathione
-
-
-
?