BRENDA - Enzyme Database

Purification and characterization of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from human liver

Uotila, L.; Biochemistry 12, 3944-3951 (1973)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.2.6
50% loss of activity on freezing and thawing
Homo sapiens
3.1.2.6
bovine serum albumin and glycerol, 30% v/v remove the sensitivity to freezing
Homo sapiens
3.1.2.6
dialysis, 50% loss of activity after 8 h
Homo sapiens
3.1.2.6
EDTA, 1 mM, labilizes the enzyme in long-term storage
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.6
2,4,6-Trinitrobenzenesulfonate
-
Homo sapiens
3.1.2.6
AsO2-
-
Homo sapiens
3.1.2.6
CdCl2
-
Homo sapiens
3.1.2.6
copper acetate
above 15 mM
Homo sapiens
3.1.2.6
diphosphate
25 mM, 50% inhibition
Homo sapiens
3.1.2.6
glutathione
weak competitive
Homo sapiens
3.1.2.6
Hg2+
HgCl2, most potent inhibitor
Homo sapiens
3.1.2.6
KCl
above 15 mM
Homo sapiens
3.1.2.6
NaCl
above 15 mM
Homo sapiens
3.1.2.6
NH4Cl
above 15 mM
Homo sapiens
3.1.2.6
oxalate
20 mM, 38% inhibition
Homo sapiens
3.1.2.6
phosphate
67 mM, 35% inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.6
0.0164
-
S-mandeloylglutathione
-
Homo sapiens
3.1.2.6
0.07
-
S-glycolylglutathione
-
Homo sapiens
3.1.2.6
0.109
-
S-glyceroylglutathione
-
Homo sapiens
3.1.2.6
0.153
-
S-formylglutathione
-
Homo sapiens
3.1.2.6
0.19
-
S-lactylglutathione
-
Homo sapiens
3.1.2.6
0.2
-
S-succinylglutathione
-
Homo sapiens
3.1.2.6
0.213
-
S-propionylglutathione
-
Homo sapiens
3.1.2.6
0.266
-
S-acetylglutathione
-
Homo sapiens
3.1.2.6
0.296
-
S-Acetoacetylglutathione
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.6
22900
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.6
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.6
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.6
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.6
822
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.6
lactoylglutathione + H2O
-
134161
Homo sapiens
D-lactate + glutathione
-
-
-
?
3.1.2.6
S-acetoacetylglutathione + H2O
55.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
acetoacetate + glutathione
-
-
-
?
3.1.2.6
S-acetylglutathione + H2O
8.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
acetate + glutathione
-
-
-
?
3.1.2.6
S-formylglutathione + H2O
37.7% of the activity with S-lactoylglutathione
134161
Homo sapiens
formate + glutathione
-
-
-
?
3.1.2.6
S-glycerylglutathione + H2O
61.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
glycerate + glutathione
-
-
-
?
3.1.2.6
S-glycoloylglutathione + H2O
38.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
glycolate + glutathione
-
-
-
?
3.1.2.6
S-mandeloylglutathione + H2O
4.8% of the activity with S-lactoylglutathione
134161
Homo sapiens
mandelate + glutathione
-
-
-
?
3.1.2.6
S-propionylglutathione + H2O
13.5% of the activity with S-lactoylglutathione
134161
Homo sapiens
propionate + glutathione
-
-
-
?
3.1.2.6
S-succinylglutathione + H2O
29.2% of the activity with S-lactoylglutathione
134161
Homo sapiens
succinate + glutathione
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.6
35
-
pH 7, 5 min, stable up to
Homo sapiens
3.1.2.6
45
-
pH 7, 5 min, 50% loss of activity
Homo sapiens
3.1.2.6
60
-
pH 7, 5 min, complete inactivation
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.6
6.8
7.5
-
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.6
25
-
diphosphate
-
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.2.6
50% loss of activity on freezing and thawing
Homo sapiens
3.1.2.6
bovine serum albumin and glycerol, 30% v/v remove the sensitivity to freezing
Homo sapiens
3.1.2.6
dialysis, 50% loss of activity after 8 h
Homo sapiens
3.1.2.6
EDTA, 1 mM, labilizes the enzyme in long-term storage
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.6
2,4,6-Trinitrobenzenesulfonate
-
Homo sapiens
3.1.2.6
AsO2-
-
Homo sapiens
3.1.2.6
CdCl2
-
Homo sapiens
3.1.2.6
copper acetate
above 15 mM
Homo sapiens
3.1.2.6
diphosphate
25 mM, 50% inhibition
Homo sapiens
3.1.2.6
glutathione
weak competitive
Homo sapiens
3.1.2.6
Hg2+
HgCl2, most potent inhibitor
Homo sapiens
3.1.2.6
KCl
above 15 mM
Homo sapiens
3.1.2.6
NaCl
above 15 mM
Homo sapiens
3.1.2.6
NH4Cl
above 15 mM
Homo sapiens
3.1.2.6
oxalate
20 mM, 38% inhibition
Homo sapiens
3.1.2.6
phosphate
67 mM, 35% inhibition
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.6
25
-
diphosphate
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.6
0.0164
-
S-mandeloylglutathione
-
Homo sapiens
3.1.2.6
0.07
-
S-glycolylglutathione
-
Homo sapiens
3.1.2.6
0.109
-
S-glyceroylglutathione
-
Homo sapiens
3.1.2.6
0.153
-
S-formylglutathione
-
Homo sapiens
3.1.2.6
0.19
-
S-lactylglutathione
-
Homo sapiens
3.1.2.6
0.2
-
S-succinylglutathione
-
Homo sapiens
3.1.2.6
0.213
-
S-propionylglutathione
-
Homo sapiens
3.1.2.6
0.266
-
S-acetylglutathione
-
Homo sapiens
3.1.2.6
0.296
-
S-Acetoacetylglutathione
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.6
22900
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.6
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.6
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.6
822
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.6
lactoylglutathione + H2O
-
134161
Homo sapiens
D-lactate + glutathione
-
-
-
?
3.1.2.6
S-acetoacetylglutathione + H2O
55.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
acetoacetate + glutathione
-
-
-
?
3.1.2.6
S-acetylglutathione + H2O
8.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
acetate + glutathione
-
-
-
?
3.1.2.6
S-formylglutathione + H2O
37.7% of the activity with S-lactoylglutathione
134161
Homo sapiens
formate + glutathione
-
-
-
?
3.1.2.6
S-glycerylglutathione + H2O
61.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
glycerate + glutathione
-
-
-
?
3.1.2.6
S-glycoloylglutathione + H2O
38.6% of the activity with S-lactoylglutathione
134161
Homo sapiens
glycolate + glutathione
-
-
-
?
3.1.2.6
S-mandeloylglutathione + H2O
4.8% of the activity with S-lactoylglutathione
134161
Homo sapiens
mandelate + glutathione
-
-
-
?
3.1.2.6
S-propionylglutathione + H2O
13.5% of the activity with S-lactoylglutathione
134161
Homo sapiens
propionate + glutathione
-
-
-
?
3.1.2.6
S-succinylglutathione + H2O
29.2% of the activity with S-lactoylglutathione
134161
Homo sapiens
succinate + glutathione
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.6
35
-
pH 7, 5 min, stable up to
Homo sapiens
3.1.2.6
45
-
pH 7, 5 min, 50% loss of activity
Homo sapiens
3.1.2.6
60
-
pH 7, 5 min, complete inactivation
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.6
6.8
7.5
-
Homo sapiens