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Literature summary extracted from

  • Razzell, W.E.; Khorana, H.G.
    Studies on polynucleotides (1961), J. Biol. Chem., 236, 1144-1149.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.16.1 analysis end group and sequential analysis of polynucleotides Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.16.1 Cu2+ strong inhibition Bos taurus
3.1.16.1 Urea 50% inhibition with 2.0 M Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.16.1 3
-
thymidine 3'-(4-nitrophenyl)phosphate
-
Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.16.1 Bos taurus
-
calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.16.1
-
Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.16.1 exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates also degradation of thymine and adenine polynucleotides to thymine and adenine Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.16.1 spleen
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.16.1 0.72
-
with polyadenylic acid as substrate Bos taurus
3.1.16.1 34
-
with thymidine 3'-(4-nitrophenyl)phosphate as substrate Bos taurus
3.1.16.1 43.3
-
with 5'-hydroxy-thymidine oligonucleotide TpT, lacking terminal phosphomonoester group, as substrate Bos taurus
3.1.16.1 43.3
-
with 5'-hydroxy-thymidine oligonucleotide TpTpTpT, lacking terminal phosphomonoester group, as substrate Bos taurus
3.1.16.1 43.3
-
with 5'-hydroxy-2'deoxy-cytosine oligonucleotide d-CpCpC, lacking terminal phosphomonoester group, as substrate Bos taurus
3.1.16.1 45
-
with 5'-hydroxy-thymidine oligonucleotide TpTp as substrate Bos taurus
3.1.16.1 58.3
-
with 5'-hydroxy-oligonucleotide ApUp as substrate Bos taurus
3.1.16.1 58.3
-
with 5'-hydroxy-adenine oligonucleotide ApApA, lacking terminal phosphomonoester group, as substrate Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.16.1 5'-hydroxy oligonucleotides + H2O 5'-hydroxy-thymidine oligonucleotides , 5'-hydroxy-adenine oligonucleotides, 5'-hydroxy-2'-deoxycytidine oligonucleotides and various other 5'-hydroxy- and 5'-hydroxy-2'deoxy oligonucleotides like ApUp, d-TpTpC Bos taurus nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
-
r
3.1.16.1 polyadenylic acid + H2O
-
Bos taurus 5'-hydroxy-adenine oligonucleotides + 3'AMP
-
r
3.1.16.1 RNA core + H2O
-
Bos taurus nucleoside 3'-phosphates
-
?
3.1.16.1 thymidine 3'-(4-nitrophenyl)phosphate + H2O
-
Bos taurus thymidine 3'-phosphate + 4-nitrophenol
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.16.1 spleen phosphodiesterase
-
Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.16.1 5.9 6.5 5.9 with almost 0.15 M ammonium succinate buffer, 6.5 with almost 0.3 M and thymidine 3'(4-nitrophenyl)phosphate as substrate Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.16.1 2
-
Urea
-
Bos taurus