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Literature summary extracted from

  • Flanagan, P.R.; Zbarsky, S.H.
    Purification of phosphodiesterase II from rat and guinea-pig intestinal mucosa (1976), Biochem. J., 155, 607-613.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.16.1 EDTA slight inhibition at 0.1 M Cavia porcellus
3.1.16.1 EDTA slight inhibition at 0.1 M Rattus norvegicus
3.1.16.1 high ionic strength slightly greater inhibition of rat than of guinea-pig enzyme at sodium succinate buffer concentrations greater 0.4 M, also markedly inhibition by 6 M NaCl and 2 M ammonium sulfate Cavia porcellus
3.1.16.1 high ionic strength slightly greater inhibition of rat than of guinea-pig enzyme at sodium succinate buffer concentrations greater 0.4 M, also markedly inhibition by 6 M NaCl and 2 M ammonium sulfate Rattus norvegicus
3.1.16.1 Mg2+ slight inbibition at 0.1 M MgCl2 Cavia porcellus
3.1.16.1 Mg2+ slight inbibition at 0.1 M MgCl2 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.16.1 0.04
-
 thymidine 3'-(2,4-dinitrophenyl)phosphate
-
 Cavia porcellus
3.1.16.1 0.04
-
 thymidine 3'-(2,4-dinitrophenyl)phosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.16.1 lysosome
-
 Cavia porcellus 5764
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.16.1 Cavia porcellus
-
-
-
3.1.16.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.16.1
-
 Cavia porcellus
3.1.16.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.16.1 intestinal mucosa homogenates of epithelial cells Cavia porcellus
-
3.1.16.1 intestinal mucosa homogenates of epithelial cells Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.16.1 0.123
-
-
 Cavia porcellus
3.1.16.1 3.45
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.16.1 thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O
-
 Cavia porcellus thymidine 3'-phosphate + 2,4-dinitrophenol
-
 ?
3.1.16.1 thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O
-
 Rattus norvegicus thymidine 3'-phosphate + 2,4-dinitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.16.1 phosphodiesterase II
-
 Cavia porcellus
3.1.16.1 phosphodiesterase II
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.16.1 61
-
 lost of 50% of activity after heating of partially purified enzyme for 5 min Cavia porcellus
3.1.16.1 64
-
 lost of 50% of activity after heating of partially purified enzyme for 5 min Rattus norvegicus