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Literature summary extracted from

  • Inada, Y.; Watanabe, H.; Ohgi, K.; Irie, M.
    Isolation, characterization, and primary structure of a base non-specific and adenylic acid preferential ribonuclease with higher specific activity from Trichoderma viride (1991), J. Biochem., 110, 896-904.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.14.1 analysis knowledge of amino acid sequences are useful for phylogenetic studies of several fungi Trichoderma viride

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.14.1 Cu2+ inhibition with 1 mM Trichoderma viride
3.1.14.1 Zn2+ inhibition with 1 mM Trichoderma viride
4.6.1.19 Cu2+
-
 Trichoderma viride
4.6.1.19 Zn2+
-
 Trichoderma viride

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.14.1 27500
-
-
 Trichoderma viride
4.6.1.19 27500
-
 ? * 27500 RNase Trv, SDS-PAGE Trichoderma viride

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O Trichoderma viride exonucleolytic cleavage 3'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Trichoderma viride
-
-
-
4.6.1.19 Trichoderma viride
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.6.1.19 glycoprotein contains 2.1% neutral sugars and 2.1 residues/mol glucosamine Trichoderma viride

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1
-
 Trichoderma viride

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.14.1 exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Trichoderma viride
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.14.1 commercial preparation
-
 Trichoderma viride
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.19 additional information
-
-
 Trichoderma viride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Trichoderma viride 3'-phosphomononucleotides
-
 ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Trichoderma viride 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Trichoderma viride 3'-phosphomononucleotides nucleotide release including 2',3'-cyclic nucloetides in decreasing order 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Trichoderma viride 3'-phosphomononucleotides preference for 3'-AMP ?
4.6.1.19 RNA + H2O
-
 Trichoderma viride 3'-phosphomononucleotides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.19 ? ? * 27500 RNase Trv, SDS-PAGE Trichoderma viride

Synonyms

EC Number Synonyms Comment Organism
3.1.14.1 RNase Trv belongs to the RNase T2 family Trichoderma viride
4.6.1.19 RNase Trv
-
 Trichoderma viride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.14.1 55
-
 with yeast RNA as substrate Trichoderma viride
4.6.1.19 55
-
-
 Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.14.1 80
-
 almost no activity Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.14.1 4.5
-
 with yeast RNA as substrate Trichoderma viride
4.6.1.19 4.5
-
-
 Trichoderma viride

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.14.1 4 10 at pH 8 stable up to at least 50°C, inactivation at higher temperatures Trichoderma viride
3.1.14.1 4 10 stable at 37°C for 1 h Trichoderma viride
4.6.1.19 4 10 37°C, 1 h Trichoderma viride