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Literature summary extracted from

  • Shetty, J.K.; Weaver, R.C.; Kinsella, J.E.
    Ribonuclease isolated from yeast (Saccharomyces carlsbergensis): characterization and properties (1981), Biotechnol. Bioeng., 23, 953-964.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.14.1 nutrition possibility for reducing nucleic acid content in yeast protein used in human food Saccharomyces pastorianus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.14.1 1,4-dithiothreitol 50% loss of activity at 5 mM, 75% at 10 mM Saccharomyces pastorianus
3.1.14.1 Ca2+ 77% of activity at 1 mM CaCl2, 73% at 10 mM Saccharomyces pastorianus
3.1.14.1 Cu2+ 73% of activity at 1 mM CuCl2, no activity at 10 mM Saccharomyces pastorianus
3.1.14.1 Fe2+ 76% of activity at 1 mM FeCl2, 46% at 10 mM Saccharomyces pastorianus
3.1.14.1 Mg2+ 91% of activity at 1 mM MgCl2, 77% at 10 mM Saccharomyces pastorianus
3.1.14.1 additional information tryptophan residues and disulfide bonds required for activity Saccharomyces pastorianus
3.1.14.1 N-bromosuccinimide
-
 Saccharomyces pastorianus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.14.1 KCl 125% of activity at 0.05 M, 138% at 0.5 M Saccharomyces pastorianus
3.1.14.1 NaCl 112% of activity at 0.05 M, 125% at 0.5 M Saccharomyces pastorianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O Saccharomyces pastorianus exonucleolytic cleavage 3'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Saccharomyces pastorianus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.1.14.1 disulfide bonds required for activity Saccharomyces pastorianus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1
-
 Saccharomyces pastorianus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.14.1 exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Saccharomyces pastorianus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides
-
 ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?

Synonyms

EC Number Synonyms Comment Organism
3.1.14.1 RNase
-
 Saccharomyces pastorianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.14.1 52
-
-
 Saccharomyces pastorianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.14.1 additional information
-
 NaCl and KCl might protect from heat denaturation at 52°C Saccharomyces pastorianus
3.1.14.1 50
-
 10 min, less than 10% loss of activity Saccharomyces pastorianus
3.1.14.1 70
-
 2 min, complete inactivation Saccharomyces pastorianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.14.1 6
-
-
 Saccharomyces pastorianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.14.1 5 10 at 2°C Saccharomyces pastorianus
3.1.14.1 5 10 at 40°C: inactivation rate at pH 10 markedly higher than at pH 4.1, 6.3 and 8.6 Saccharomyces pastorianus