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Literature summary extracted from

  • Shetty, J.K.; Weaver, R.C.; Kinsella, J.E.
    A rapid method for the isolation of ribonuclease from yeast (Saccharomyces carlsbergensis) (1980), Biochem. J., 189, 363-366.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.14.1 EDTA 1 mM: inhibition of intracellular proteolytic proteins during the incubation of disrupted yeast cells for RNA hydrolysis Saccharomyces pastorianus
3.1.14.1 phenylmethanesulfonyl fluoride 0.1 mM: inhibition of intracellular proteolytic proteins during the incubation of disrupted yeast cells for RNA hydrolysis Saccharomyces pastorianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O Saccharomyces pastorianus exonucleolytic cleavage 3'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Saccharomyces pastorianus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1
-
 Saccharomyces pastorianus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.14.1 exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Saccharomyces pastorianus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.14.1 additional information released from disrupted cells Saccharomyces pastorianus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.14.1 additional information
-
-
 Saccharomyces pastorianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides
-
 ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides no base specificity ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?

Synonyms

EC Number Synonyms Comment Organism
3.1.14.1 RNase
-
 Saccharomyces pastorianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.14.1 52
-
-
 Saccharomyces pastorianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.14.1 6
-
-
 Saccharomyces pastorianus