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Literature summary extracted from
- Partial purification and some properties of a T2 ribonuclease from Aspergillus flaviceps (1996), Microbios, 87, 227-237.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.14.1 | EDTA | 120% of maximal activity with 1 mM | Aspergillus flavipes |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.14.1 | Ag+ | no activity at 1 mM AgNO3 | Aspergillus flavipes | |
| 3.1.14.1 | Ba2+ | 85% of maximal activity at 1 mM BaCl2 | Aspergillus flavipes | |
| 3.1.14.1 | Cd2+ | 75% of maximal activity at 1 mM CdCl2 | Aspergillus flavipes | |
| 3.1.14.1 | Co2+ | 82% of maximal activity at 1 mM CoCl2 | Aspergillus flavipes | |
| 3.1.14.1 | Cu2+ | 63% of maximal activity at 1 mM CuSO4 | Aspergillus flavipes | |
| 3.1.14.1 | Fe3+ | 41% of maximal activity at 1 mM FeCl3 | Aspergillus flavipes | |
| 3.1.14.1 | Hg2+ | no activity at 1 mM HgCl2 | Aspergillus flavipes | |
| 3.1.14.1 | Mn2+ | 85% of maximal activity at 1 mM MnSO4 | Aspergillus flavipes | |
| 3.1.14.1 | Zn2+ | 55% of maximal activity at 1 mM ZnCl2 | Aspergillus flavipes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 40000 | - |
- |
Aspergillus flavipes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.14.1 | Aspergillus flavipes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.14.1 | partial purification | Aspergillus flavipes |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.14.1 | exonucleolytic cleavage to nucleoside 3'-phosphates | exonucleolytic cleavage | Aspergillus flavipes |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 11.3 | - |
179fold partial purification | Aspergillus flavipes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.14.1 | ribonucleic acid + H2O | exonucleolytic cleavage | Aspergillus flavipes | 3'-phosphomononucleotides | no base specificity | ? | |
| 3.1.14.1 | ribonucleic acid + H2O | exonucleolytic cleavage | Aspergillus flavipes | 3'-phosphomononucleotides | preference for 3'-AMP | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.14.1 | Afl II ribonuclease | - |
Aspergillus flavipes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 50 | 55 | sodium acetate buffer at pH 4.0 | Aspergillus flavipes |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 60 | - |
remaining activity after heating at 60°C for 90 min at pH 5.4 30%, at pH 7.0 40%, at pH 9.0 50% | Aspergillus flavipes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 4 | 4.25 | 0.25 M sodium acetate buffer | Aspergillus flavipes |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 3 | 9 | 50% of activity near pH 5.0 | Aspergillus flavipes |
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Release 2023.1 (January 2023)
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