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Literature summary extracted from
- Ribonuclease U4. Novel phosphotransferases catalyzing exonucleolytic degradation of ribonucleic acid (1972), Biochemistry, 11, 3962-3970.
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.14.1 | ribonucleic acid + H2O | Ustilago sphaerogena | exonucleolytic cleavage | 3'-phosphomononucleotides | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.14.1 | Ustilago sphaerogena | - |
separable components RNase U4A, U4B, U4C | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.14.1 | exonucleolytic cleavage to nucleoside 3'-phosphates | exonucleolytic cleavage | Ustilago sphaerogena |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.14.1 | culture medium | - |
Ustilago sphaerogena | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.14.1 | additional information | only slight degradation of denaturated DNA, p-nitrophenyl phosphate, bis(p-nitrophenyl)phosphate | Ustilago sphaerogena | ? | - |
? | |
| 3.1.14.1 | ribonucleic acid + H2O | exonucleolytic cleavage | Ustilago sphaerogena | 3'-phosphomononucleotides | - |
? | |
| 3.1.14.1 | ribonucleic acid + H2O | exonucleolytic cleavage | Ustilago sphaerogena | 3'-phosphomononucleotides | 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP | ? | |
| 3.1.14.1 | ribonucleic acid + H2O | exonucleolytic cleavage | Ustilago sphaerogena | 3'-phosphomononucleotides | 2',3' cyclic mononucleotides | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.14.1 | ribonuclease U4 | - |
Ustilago sphaerogena |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 6 | 7 | U4A: 7.0 | Ustilago sphaerogena |
| 3.1.14.1 | 6 | 7 | U4C: 6.0 | Ustilago sphaerogena |
| 3.1.14.1 | 6 | 7 | U4B: 6.5 | Ustilago sphaerogena |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.14.1 | 5 | 8 | 0.04 M sodium phosphate buffer | Ustilago sphaerogena |
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Release 2023.1 (January 2023)
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