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Literature summary extracted from

  • Blank, A.; Dekker, C.A.
    Ribonuclease U4 from Ustilago sphaerogena. Purification and physical properties (1972), Biochemistry, 11, 3956-3962.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.14.1 20000 23000 gel filtration Ustilago sphaerogena
3.1.14.1 20000 23000 207000 for RNase U4A, 21300 for RNase U4B, 22100 RNase U4C Ustilago sphaerogena
3.1.14.1 26000 29000 equilibrium ultracentrifugation Ustilago sphaerogena
3.1.14.1 26000 29000 29500 for RNase U4A, 26900 for RNase U4B, 20000for RNase U4C Ustilago sphaerogena

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O Ustilago sphaerogena exonucleolytic cleavage 3'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Ustilago sphaerogena
-
 separable components RNase U4A, U4B, U4C
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1
-
 Ustilago sphaerogena

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.14.1 exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Ustilago sphaerogena
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.14.1 culture medium
-
 Ustilago sphaerogena
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Ustilago sphaerogena 3'-phosphomononucleotides
-
 ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Ustilago sphaerogena 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?

Synonyms

EC Number Synonyms Comment Organism
3.1.14.1 ribonuclease U4
-
 Ustilago sphaerogena