Literature summary extracted from
Ohtaka, Y.; Uchida, K.; Sakai, T.
Purification and properties of ribonuclease from yeast (1963), J. Biochem., 54, 322-327.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.1.14.1 |
EDTA |
slight activation |
Saccharomyces cerevisiae |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.14.1 |
Cd2+ |
100% inhibition by 0.01 M CdCl2 |
Saccharomyces cerevisiae |
|
3.1.14.1 |
Co2+ |
100% inhibition by 0.01 M CoCl2 |
Saccharomyces cerevisiae |
|
3.1.14.1 |
Cu2+ |
50% inhibition by 1 mM CuSO4 |
Saccharomyces cerevisiae |
|
3.1.14.1 |
glutathione |
95% inhibition by 0.01 M |
Saccharomyces cerevisiae |
|
3.1.14.1 |
Mn2+ |
20% of maximal activity |
Saccharomyces cerevisiae |
|
3.1.14.1 |
Zn2+ |
100% inhibition by 0.1 mM ZnSO4, reversible by addition of 1 mM EDTA, sodium citrate and histidine, not by glycine, arginine, agmatine and sodium acetate even at 0.01 M |
Saccharomyces cerevisiae |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.14.1 |
KCl |
slight activation |
Saccharomyces cerevisiae |
|
3.1.14.1 |
KH2PO4 |
10-80% activation at various concentrations |
Saccharomyces cerevisiae |
|
3.1.14.1 |
Na2SO4 |
slight activation |
Saccharomyces cerevisiae |
|
3.1.14.1 |
NaCl |
slight activation |
Saccharomyces cerevisiae |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.14.1 |
ribonucleic acid + H2O |
Saccharomyces cerevisiae |
exonucleolytic cleavage |
3'-phosphomononucleotides |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.14.1 |
Saccharomyces cerevisiae |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.14.1 |
- |
Saccharomyces cerevisiae |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.1.14.1 |
exonucleolytic cleavage to nucleoside 3'-phosphates |
exonucleolytic cleavage |
Saccharomyces cerevisiae |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.14.1 |
additional information |
released from pressed cells |
Saccharomyces cerevisiae |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.14.1 |
additional information |
- |
- |
Saccharomyces cerevisiae |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
3.1.14.1 |
4°C, under toluene, pH 7.5, several weeks, little loss of activity |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.14.1 |
additional information |
not: DNA, diphenylphosphate, 2',3'-cyclic nucleotides |
Saccharomyces cerevisiae |
? |
- |
? |
|
3.1.14.1 |
ribonucleic acid + H2O |
exonucleolytic cleavage |
Saccharomyces cerevisiae |
3'-phosphomononucleotides |
- |
? |
|
3.1.14.1 |
ribonucleic acid + H2O |
exonucleolytic cleavage |
Saccharomyces cerevisiae |
3'-phosphomononucleotides |
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.14.1 |
50 |
- |
30 min reaction time |
Saccharomyces cerevisiae |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.1.14.1 |
35 |
65 |
less than 15% of maximum activity above and below |
Saccharomyces cerevisiae |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.1.14.1 |
40 |
- |
several h under toluene, pH 7.5 |
Saccharomyces cerevisiae |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.14.1 |
7.5 |
- |
- |
Saccharomyces cerevisiae |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.1.14.1 |
4.5 |
9 |
Ph 4.5: about 15% of maximum activity, pH 9.0: about 50% of maximum activity |
Saccharomyces cerevisiae |