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Literature summary extracted from

  • Sporn, M.B.; Lazarus, H.M.; Smith, J.M.; Henderson, W.R.
    Studies on nuclear exoribonucleases. 3. Isolation and properties of the enzyme from normal and malignant tissues of the mouse (1969), Biochemistry, 8, 1698-1706.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.1 RNA with a 2',3'-cyclic phosphate group on the potential substrate molecule Mus musculus
3.1.13.1 RNA with a terminal 3'-phosphate group on the potential substrate molecule Mus musculus
3.1.13.1 thymidine nucleotides potent inhibitor, pTp at a concentration of 0.00004 M inhibits degradation of poly(A) by 60%, pTpT at a concentration of 0.0005 M inhibits degradation of poly(A) by 30% Mus musculus
3.1.13.1 Urea loss of 40% of activity in presence of 1.6 M, 60% in presence of 2.4 M and 75% in presence of 3.2 M urea Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.13.1 nucleus
-
 Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.1 ss RNA + H2O Mus musculus
-
 5'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.1 Mus musculus
-
 mouse
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.13.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.13.1 breast adenocarcinoma cell
-
 Mus musculus
-
3.1.13.1 Ehrlich ascites carcinoma cell
-
 Mus musculus
-
3.1.13.1 embryo
-
 Mus musculus
-
3.1.13.1 kidney
-
 Mus musculus
-
3.1.13.1 liver
-
 Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
3.1.13.1 -70°C, more than 50% of activity after more than a year Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.1 additional information 3'-phosphorylated RNA is not hydrolyzed Mus musculus ?
-
 ?
3.1.13.1 poly(A) + H2O
-
 Mus musculus 5'-AMP + oligonucleotide
-
 ?
3.1.13.1 ss RNA + H2O
-
 Mus musculus 5'-phosphomononucleotides
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.13.1 50
-
 in 0.1 M Tris-Cl, pH 8.0, for 15 min, loss of 20-30% of activity Mus musculus
3.1.13.1 50
-
 in 0.1 M Tris-Cl, pH 8.0, for 60 min, loss of 40-60% of activity Mus musculus