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Literature summary extracted from

  • Ning Kwan, C.
    A cytoplasmic exoribonuclease from HeLa cells (1977), Biochim. Biophys. Acta, 479, 322-331.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.1 NaCl a salt concentration of 0.05 M inhibits 50% of the activity, salt optimum: 0.01 M Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.13.1 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.1 Mg2+ necessary for activity Homo sapiens
3.1.13.1 Mg2+ optimum: 1 mM Homo sapiens
3.1.13.1 Mn2+ can partially replace Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.1 ss RNA + H2O Homo sapiens
-
 5'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.1 partial purification, 500-700fold Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.13.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.13.1 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.13.1 0.004
-
 substrate: poly(A) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.1 additional information ds RNA is not hydrolyzed Homo sapiens ?
-
 ?
3.1.13.1 additional information ds DNA is not hydrolyzed Homo sapiens ?
-
 ?
3.1.13.1 mRNA + H2O
-
 Homo sapiens 5'-phosphomononucleotides
-
 ?
3.1.13.1 poly(A) + H2O
-
 Homo sapiens 5'-AMP + oligonucleotide
-
 ?
3.1.13.1 poly(C) + H2O
-
 Homo sapiens 5'-CMP + oligonucleotide
-
 ?
3.1.13.1 ss RNA + H2O
-
 Homo sapiens 5'-phosphomononucleotides
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.13.1 60
-
 for 1 min completely destroys enzyme activity Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.13.1 0.05
-
 NaCl salt 0.01 M Homo sapiens