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Literature summary extracted from

  • Gupta, R.S.; Kasai, T.; Schlessinger, D.
    Purification and some novel properties of Escherichia coli RNase II (1977), J. Biol. Chem., 252, 8945-8949.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.1 Na+ substrate: artificial polynucleotides Escherichia coli
3.1.13.1 Poly(U) strong competitive inhibitor of T4 mRNA-degradation Escherichia coli
3.1.13.1 rRNA weak inhibitor of T4 mRNA-degradation Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.1 K+ activation against poly(A), -(U), -(C), RNA and T4 mRNA Escherichia coli
3.1.13.1 Li+ can substitute for K+ to a small extend Escherichia coli
3.1.13.1 Na+ increases activity against T4 mRNA, can substitute for K+ in activation against T4 mRNA and RNA Escherichia coli
3.1.13.1 NH4+ activating Escherichia coli
3.1.13.1 NH4+ can substitute for K+ to a small extend Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.13.1 80000 90000 native and SDS-PAGE, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.1 ss RNA + H2O Escherichia coli
-
 5'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.1 to apparent homogeneity, 270fold Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.13.1 0.04
-
 activity of the most active fraction, substrate: T4 mRNA Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.13.1 -20°C, small aliquots of purified enzyme, 10% glycerol, stable for at least 19 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.1 ss RNA + H2O
-
 Escherichia coli 5'-phosphomononucleotides
-
 ?
3.1.13.1 T4 mRNA + H2O
-
 Escherichia coli 5'-phosphomononucleotides
-
 ?