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Literature summary extracted from

  • Chase, J.W.; Vales, L.D.
    Exonuclease VII of E. coli (1981), Gene Amplif. Anal., 2, 147-168.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.11.6 analysis degradation of adenovirus-associated DNA Escherichia coli
3.1.11.6 analysis of nucleic acid structure Escherichia coli
3.1.11.6 analysis removal of single-stranded DNA Escherichia coli
3.1.11.6 analysis of spliced RNA Escherichia coli
3.1.11.6 analysis excision of DNA segments from cloning vectors Escherichia coli

General Stability

EC Number General Stability Organism
3.1.11.6 freezing and thawing causes loss of activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.6 single-stranded DNA + H2O Escherichia coli
-
 hydrolyzed single-stranded DNA oligonucleotides ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.6 Escherichia coli
-
 xseA
-
3.1.11.6 Escherichia coli
-
 numerous mutants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.6 1700fold Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.11.6 -20°C, 20 mM Tris-HCl, pH 8, 10 mM mercaptoethanol, 0.1 mM EDTA, 0.2 M NaCl, 50% glycerol, 18 months, 10% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.6 single-stranded DNA + H2O
-
 Escherichia coli hydrolyzed single-stranded DNA oligonucleotides ?
3.1.11.6 single-stranded DNA + H2O processive degradation Escherichia coli hydrolyzed single-stranded DNA
-
 ?
3.1.11.6 thymine dimers + H2O possible role in repair of ultraviolet damage Escherichia coli hydrolyzed single-stranded DNA
-
 ?