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Literature summary extracted from

  • Masterson, C.; Boehmer, P.E.; McDonald, F.; Chaudhuri, S.; Hickson, I.D.; Emmerson, P.T.
    Reconstitution of the activities of the RecBCD holoenzyme of Escherichia coli from the purified subunits (1992), J. Biol. Chem., 267, 13564-13572.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.11.5 K177Q involved in ATP binding site of the recD protein Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.5 333000
-
 gel filtration, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 ATP + H2O Escherichia coli
-
 ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent helicase single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O Escherichia coli ATP-dependent endonuclease 5'-phosphomonoester oligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.5 of the recombinant proteins B, C and D Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O individual RecB protein has ATPase activity Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA loop is formed on the same strand that is cut by the enzyme near chi Escherichia coli intermediates with single stranded regions
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent helicase Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O also endonuclease activity on circular DNA Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP-dependent endonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.5 trimer
-
 Escherichia coli