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Literature summary extracted from
- Exonuclease V from Saccharomyces cerevisiae. A 5-3-deoxyribonuclease that produces dinucleotides in a sequential fashion (1988), J. Biol. Chem., 263, 8099-8105.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.11.5 | NaCl | - |
Saccharomyces cerevisiae |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.11.5 | 0.007 | - |
oligonucleotide (polydT)8 polydA | - |
Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.11.5 | nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.11.5 | Mg2+ | required, optimal concentration around 3-12 mM | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.11.5 | 99000 | - |
sedimentation, calculated | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.11.5 | single stranded DNA + H2O | Saccharomyces cerevisiae | - |
5'-phosphomonoester oligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.11.5 | Saccharomyces cerevisiae | - |
yeast | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.11.5 | partial | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.11.5 | oligonucleotide (polydT)8 polydA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.1.11.5 | single stranded DNA + H2O | - |
Saccharomyces cerevisiae | 5'-phosphomonoester oligonucleotides | - |
? | |
| 3.1.11.5 | single stranded DNA + H2O | no endonuclease activity | Saccharomyces cerevisiae | 5'-phosphomonoester oligonucleotides | - |
? | |
| 3.1.11.5 | single stranded DNA + H2O | degrades only single stranded DNA from 5'-end | Saccharomyces cerevisiae | 5'-phosphomonoester oligonucleotides | - |
? | |
| 3.1.11.5 | single stranded DNA + H2O | not ATP dependent | Saccharomyces cerevisiae | 5'-phosphomonoester oligonucleotides | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.11.5 | 7.8 | - |
- |
Saccharomyces cerevisiae |
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