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Literature summary extracted from

  • Carter, D.M.; Radding, C.M.
    The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease (1971), J. Biol. Chem., 246, 2502-2512.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.3 24000
-
gel fitration, SDS-PAGE Escherichia coli
3.1.11.3 24000
-
gel fitration, SDS-PAGE Lambdavirus lambda
3.1.11.3 28000
-
alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea Escherichia coli
3.1.11.3 28000
-
alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea Lambdavirus lambda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O Escherichia coli
-
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
?
3.1.11.3 double-stranded DNA + H2O Lambdavirus lambda
-
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
?
3.1.11.3 single-stranded DNA + H2O Escherichia coli
-
5'-phosphomononucleotides
-
?
3.1.11.3 single-stranded DNA + H2O Lambdavirus lambda
-
5'-phosphomononucleotides
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.3 Escherichia coli
-
Escherichia coli infected with phage lambda only
-
3.1.11.3 Lambdavirus lambda
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.3
-
Escherichia coli
3.1.11.3
-
Lambdavirus lambda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O
-
Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
?
3.1.11.3 double-stranded DNA + H2O
-
Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
?
3.1.11.3 double-stranded DNA + H2O no attack at single-stranded breaks within DNA-duplex Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
?
3.1.11.3 double-stranded DNA + H2O no attack at single-stranded breaks within DNA-duplex Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
?
3.1.11.3 single-stranded DNA + H2O
-
Escherichia coli 5'-phosphomononucleotides
-
?
3.1.11.3 single-stranded DNA + H2O
-
Lambdavirus lambda 5'-phosphomononucleotides
-
?

Subunits

EC Number Subunits Comment Organism
3.1.11.3 dimer alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea Escherichia coli
3.1.11.3 dimer alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea Lambdavirus lambda

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.11.3 7.5
-
no activity at DNA nicks Escherichia coli
3.1.11.3 7.5
-
no activity at DNA nicks Lambdavirus lambda
3.1.11.3 9.6
-
no activity at DNA nicks Escherichia coli
3.1.11.3 9.6
-
no activity at DNA nicks Lambdavirus lambda