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Literature summary extracted from

  • Cassuto, E.; Radding, C.M.
    Mechanism for the action of lambda exonuclease in genetic recombination (1971), Nature New Biol., 229, 13-16.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O Escherichia coli
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O Lambdavirus lambda
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O Escherichia coli at single-stranded DNA branches 5'-phosphomononucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O Lambdavirus lambda at single-stranded DNA branches 5'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.3 Escherichia coli
-
 Escherichia coli infected with phage lambda only
-
3.1.11.3 Lambdavirus lambda
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O
-
 Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O
-
 Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O mechanism for stimulation of recombination Escherichia coli 5'-phosphomononucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O mechanism for stimulation of recombination Lambdavirus lambda 5'-phosphomononucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O degrades the 5'-terminated strand at a single-stranded branch Escherichia coli 5'-phosphomononucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O degrades the 5'-terminated strand at a single-stranded branch Lambdavirus lambda 5'-phosphomononucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O at single-stranded DNA branches Escherichia coli 5'-phosphomononucleotides
-
 ?
3.1.11.3 single-stranded DNA + H2O at single-stranded DNA branches Lambdavirus lambda 5'-phosphomononucleotides
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.11.3 6
-
 Double-stranded DNA at 20°C and pH 7.5 Escherichia coli
3.1.11.3 6
-
 Double-stranded DNA at 20°C and pH 7.5 Lambdavirus lambda
3.1.11.3 11.7
-
 Double-stranded DNA at 37°C and pH 7.5 Escherichia coli
3.1.11.3 11.7
-
 Double-stranded DNA at 37°C and pH 7.5 Lambdavirus lambda