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Literature summary extracted from

  • Prasher, D.C.; Conarro L.; Kushner, S.R.
    Amplification and purification of exonuclease I from Escherichia coli (1983), J. Biol. Chem., 2558, 6340-6343.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.11.1 expression in Escherichia coli Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.1 55000
-
 SDS-PAGE, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.1 single-stranded polydeoxyribonucleotide + H2O Escherichia coli
-
 single-stranded oligodeoxynucleotide + nucleoside 5'-monophosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.1 overexpression in Escherichia coli, chromatography techniques, near homogeneity Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.11.1 cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.11.1 0.018
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.1 single-stranded polydeoxyribonucleotide + H2O
-
 Escherichia coli single-stranded oligodeoxynucleotide + nucleoside 5'-monophosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.1 monomer 1 * 55000, SDS-PAGE, gel filtration Escherichia coli