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Literature summary extracted from

  • Rahman, Y.E.; Cerny, E.A.; Peraino, C.
    Purification and some properties of rat spleen phospholipase A (1973), Biochim. Biophys. Acta, 321, 526-535.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 Cu2+
-
 Rattus norvegicus
3.1.1.4 deoxycholate substrate: phosphatidylcholine Rattus norvegicus
3.1.1.4 EDTA
-
 Rattus norvegicus
3.1.1.4 EDTA
-
 Sus scrofa
3.1.1.4 Fe2+
-
 Rattus norvegicus
3.1.1.4 Hg2+
-
 Rattus norvegicus
3.1.1.4 Mg2+
-
 Rattus norvegicus
3.1.1.4 Triton X-100
-
 Rattus norvegicus
3.1.1.4 Zn2+
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Rattus norvegicus
3.1.1.4 Ca2+
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 15000
-
-
 Sus scrofa
3.1.1.4 15000
-
 gel filtration, gel electrophoresis, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Rattus norvegicus
-
 ?
-
 ?
3.1.1.4 phospholipids + H2O Sus scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Rattus norvegicus
-
-
-
3.1.1.4 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 pancreas
-
 Sus scrofa
-
3.1.1.4 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 5000
-
 substrate: phosphatidylethanolamine Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus 1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Rattus norvegicus 1-acylglycerophosphocholine + fatty acid
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Sus scrofa 1-acylglycerophosphocholine + fatty acid
-
 ?
3.1.1.4 phosphatidylethanolamine + H2O
-
 Rattus norvegicus 1-acylglycerophosphorylethanolamine + fatty acid
-
 ?
3.1.1.4 phosphatidylethanolamine + H2O
-
 Sus scrofa 1-acylglycerophosphorylethanolamine + fatty acid
-
 ?
3.1.1.4 phospholipids + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.1.4 phospholipids + H2O
-
 Sus scrofa ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 60
-
 for 10 min at pH 5.0 no loss of activity, at pH 7.0 50% of activity remains, pH 9.0 leads to complete loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 7
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.4 6 9
-
 Rattus norvegicus