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Literature summary extracted from

  • Donne-Op den Kelder, G.M.; de Haas, G.H.; Egmond, M.R.
    Localization of the second calcium ion binding site in porcine and equine phospholipase A2 (1983), Biochemistry, 22, 2470-2478.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ two Ca2+ binding sites, first Ca2+ is strongly bound and essential for enzyme activity, the second binds more weakly and improves the enzymes affinity for lipid-water interfaces Homo sapiens
3.1.1.4 Ca2+ two Ca2+ binding sites, first Ca2+ is strongly bound and essential for enzyme activity, the second binds more weakly and improves the enzymes affinity for lipid-water interfaces Sus scrofa
3.1.1.4 Ca2+ two Ca2+ binding sites, first Ca2+ is strongly bound and essential for enzyme activity, the second binds more weakly and improves the enzymes affinity for lipid-water interfaces Bos taurus
3.1.1.4 Ca2+ two Ca2+ binding sites, first Ca2+ is strongly bound and essential for enzyme activity, the second binds more weakly and improves the enzymes affinity for lipid-water interfaces Equus caballus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 13750
-
 amino acid composition analysis Bos taurus
3.1.1.4 13900
-
 amino acid composition analysis Homo sapiens
3.1.1.4 13900
-
 amino acid composition analysis Equus caballus
3.1.1.4 13950
-
 amino acid composition analysis Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Homo sapiens
-
 ?
-
 ?
3.1.1.4 phospholipids + H2O Sus scrofa
-
 ?
-
 ?
3.1.1.4 phospholipids + H2O Bos taurus
-
 ?
-
 ?
3.1.1.4 phospholipids + H2O Equus caballus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Bos taurus
-
-
-
3.1.1.4 Equus caballus
-
-
-
3.1.1.4 Homo sapiens
-
-
-
3.1.1.4 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4
-
 Homo sapiens
3.1.1.4
-
 Sus scrofa
3.1.1.4
-
 Bos taurus
3.1.1.4
-
 Equus caballus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 pancreas
-
 Homo sapiens
-
3.1.1.4 pancreas
-
 Sus scrofa
-
3.1.1.4 pancreas
-
 Bos taurus
-
3.1.1.4 pancreas
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 573
-
 pH 8, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.013 mM Ca2+ Homo sapiens
3.1.1.4 1648
-
 pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, isoenzyme of e-amidinated PLA, 0.71 mM Ca2+ Sus scrofa
3.1.1.4 1693
-
 pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.42 mM Ca2+ Homo sapiens
3.1.1.4 2038
-
 pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.64 mM Ca2+ Sus scrofa
3.1.1.4 2170
-
 pH8, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, iso-AMPA, 0.30 mM Ca2+ Sus scrofa
3.1.1.4 2725
-
 pH8, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 2.0 mM Ca2+ Bos taurus
3.1.1.4 2910
-
 pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 8.1 mM Ca2+ Bos taurus
3.1.1.4 6671
-
 pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.79 mM Ca2+ Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Sus scrofa 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Bos taurus 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Equus caballus 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 phospholipids + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O
-
 Sus scrofa ?
-
 ?
3.1.1.4 phospholipids + H2O
-
 Bos taurus ?
-
 ?
3.1.1.4 phospholipids + H2O
-
 Equus caballus ?
-
 ?