Literature summary extracted from

Hoffman, W.J.; Vahey, M.; Hajdu, J.
Pancreatic porcine phospholipase A2 catalyzed hydrolysis of phosphatidylcholine in lecithin-bile salt mixed micelles: Kinetic studies in a lecithin-sodium cholate system (1983), Arch. Biochem. Biophys., 221, 361-370.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.1.1.4	additional information	mixed micelle required to bind to the enzyme before the substrate, not altered by reaction	Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
3.1.1.4	phospholipids + H2O	Naja naja	-	?	-	?
3.1.1.4	phospholipids + H2O	Sus scrofa	aldosterone-dependent sodium transport	?	-	?
3.1.1.4	phospholipids + H2O	Sus scrofa	cardiac contraction and excitation	?	-	?
3.1.1.4	phospholipids + H2O	Sus scrofa	release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.4	Naja naja	-	cobra	-
3.1.1.4	Sus scrofa	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.1.4	pancreas	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.1.4	1	10	phospholipid/Triton X-100: 1/2,1/3,und 1/4, phospholipid concentration up to 30 mM	Sus scrofa
3.1.1.4	82	-	5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.20	Sus scrofa
3.1.1.4	150	-	5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.36	Sus scrofa
3.1.1.4	330	-	freshly dialyzed PLA2	Sus scrofa
3.1.1.4	383	-	5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67	Sus scrofa
3.1.1.4	460	-	7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67	Sus scrofa
3.1.1.4	520	-	10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67	Sus scrofa
3.1.1.4	550	-	12.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67	Sus scrofa
3.1.1.4	550	-	15 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 0.67	Sus scrofa
3.1.1.4	576	-	5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0	Sus scrofa
3.1.1.4	741	-	5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42	Sus scrofa
3.1.1.4	775	-	7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0	Sus scrofa
3.1.1.4	804	-	10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0	Sus scrofa
3.1.1.4	870	-	7.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42	Sus scrofa
3.1.1.4	900	-	12.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0	Sus scrofa
3.1.1.4	900	-	15 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.0	Sus scrofa
3.1.1.4	933	-	5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 2.0	Sus scrofa
3.1.1.4	1050	-	10 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42	Sus scrofa
3.1.1.4	1100	-	12.5 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42	Sus scrofa
3.1.1.4	1100	-	phospholipid/Triton X-100: 1/4	Naja naja
3.1.1.4	1100	-	15 mM phosphocholine, N-phosphocholine/N-sodiumcholate: 1.42	Sus scrofa
3.1.1.4	1670	-	phospholipid/Triton X-100: 1/3	Naja naja
3.1.1.4	2100	-	phospholipid/Triton X-100: 1/2	Naja naja

Storage Stability

EC Number	Storage Stability	Organism
3.1.1.4	4°C, pH 7.5, 0.05 M sodium phosphate buffer, no loss of activity over a period of several months	Naja naja

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3.1.1.4	phosphatidylcholine + H2O	-	Sus scrofa	1-acylglycerophosphocholine + fatty acid	-	?
3.1.1.4	phospholipids + H2O	-	Naja naja	?	-	?
3.1.1.4	phospholipids + H2O	aldosterone-dependent sodium transport	Sus scrofa	?	-	?
3.1.1.4	phospholipids + H2O	cardiac contraction and excitation	Sus scrofa	?	-	?
3.1.1.4	phospholipids + H2O	release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins	Sus scrofa	?	-	?