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Literature summary extracted from

  • Paglin, S.; Roy, R.; Polgar, P.
    Characterization of hormonally regulated and particulate-associated phospholipase A2 from bovine endothelial cells (1993), J. Biol. Chem., 268, 11697-11702.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.4 octyl glucoside, selective solubilization of hormonally regulated PLA2, stable Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 cytoplasm
-
 Bos taurus 5737
-
3.1.1.4 particle-bound
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 103000
-
 centrifugation in sucrose density gradients Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Bos taurus influence on platelet aggregation ?
-
 ?
3.1.1.4 phospholipids + H2O Bos taurus release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 subcellular fractionation Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 endothelial cell from pulmonary artery Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 0.0000115
-
 particulate associated hormonally regulated PLA2, substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine, mixed micelles with Triton X-100 Bos taurus
3.1.1.4 0.0000538
-
 particulate associated hormonally regulated PLA2, substrate: micellar 1-palmitoyl-2-arachidonylphosphatidylcholine Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 -70°C, for up to 2 weeks without loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
 Bos taurus 1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
 ?
3.1.1.4 1-palmitoyl-2-oleoylphosphatidylcholine + H2O
-
 Bos taurus 1-palmitoyl-glycerophosphocholine + oleic acid
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Bos taurus ?
-
 ?
3.1.1.4 phospholipids + H2O release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 7.5 8.5 higher activity at pH 8.5, barely detectable activity at pH 5 or 6 Bos taurus