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Literature summary extracted from

  • Han, S.K.; Yoon, E.T.; Cho, W.
    Bacterial expression and characterization of human secretory class V phospholipase A2 (1998), Biochem. J., 331, 353-357.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 into pET21a vector, transformation of Escherichia coli strain BL21(DE3) Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 secretory granule
-
 Homo sapiens 30141
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 14000
-
 heparin-sepharose affinity chromatography, SDS-PAGE after digestion with thrombin, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Homo sapiens eicosanoids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 gel filtration chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 -4°C, more than 2 weeks without precipitation Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphatidic acid + H2O group V sPLA2 Homo sapiens ?
-
 ?
3.1.1.4 1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphocholine + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphocholine + 1-pyrenedecanoate
-
 ?
3.1.1.4 1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoethanolamine + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphoethanolamine + 1-pyrenedecanoate
-
 ?
3.1.1.4 1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoglycerol + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphoglycerol + 1-pyrenedecanoate
-
 ?
3.1.1.4 1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoserine + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphoserine + 1-pyrenedecanoate
-
 ?
3.1.1.4 phospholipids + H2O eicosanoids Homo sapiens ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 70
-
 group V sPLA2 shows a half-life for inactivation of 10 min., group IIa sPLA2 retains 75% of activity after 1 h Homo sapiens