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Literature summary extracted from

  • Pan, F.M.; Chao, S.C.; Wu, S.H.; Chang, W.C.; Chiou, S.H.
    Characterization of phospholipase A2 (PLA2) from Taiwan Cobra: Isoenzymes and their site-directed mutants (1998), Biochem. Biophys. Res. Commun., 250, 154-160.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 into pQE-30 of Escherichia coli Naja atra

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.4 D97N reduced activity of acidic secretory isoforms PLA2-1 and PLA2-2 Naja atra
3.1.1.4 H47A no activity of acidic secretory isoforms PLA2-1 and PLA2-2 Naja atra
3.1.1.4 H47R no activity of acidic secretory isoforms PLA2-1 and PLA2-2 Naja atra

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 secretory granule
-
 Naja atra 30141
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 13220
-
 PLA2-2, SDS-PAGE and mass spectrometry Naja atra
3.1.1.4 13260
-
 PLA2-1, SDS-PAGE and mass spectrometry Naja atra

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Naja atra myotoxicity ?
-
 ?
3.1.1.4 phospholipids + H2O Naja atra presynaptic neurotoxicity of neutral PLA2 ?
-
 ?
3.1.1.4 phospholipids + H2O Naja atra cardiac toxicity ?
-
 ?
3.1.1.4 phospholipids + H2O Naja atra hemolytic activity of basic PLA2 ?
-
 ?
3.1.1.4 phospholipids + H2O Naja atra influence on platelet aggregation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Naja atra
-
 Taiwan cobra
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 Ni-NTA affinity chromatography Naja atra

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4
-
 Naja atra

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 venom
-
 Naja atra
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Naja atra 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 phospholipids + H2O myotoxicity Naja atra ?
-
 ?
3.1.1.4 phospholipids + H2O presynaptic neurotoxicity of neutral PLA2 Naja atra ?
-
 ?
3.1.1.4 phospholipids + H2O cardiac toxicity Naja atra ?
-
 ?
3.1.1.4 phospholipids + H2O hemolytic activity of basic PLA2 Naja atra ?
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Naja atra ?
-
 ?