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Literature summary extracted from

  • Liu, X.; Pan, H.; Yang, G.; Wu, X.; Zhou, Y.
    Cloning, expression and biochemical characterization of a basic-acidic hybrid phospholipase A2-II from Agkistrodon halys Pallas (1999), Biochim. Biophys. Acta, 1431, 157-165.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 basic-acidic hybrid PLA2 cDNA, cloned into the expression vector pBLMVL2 for later transformation in Escherichia coli Gloydius halys

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.4 acidic and basic PLA2 Gloydius halys

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phosphatidylcholine + H2O Gloydius halys
-
 lysophosphatidylcholine + a fatty acid
-
 ?
3.1.1.4 phospholipids + H2O Gloydius halys myotoxicity ?
-
 ?
3.1.1.4 phospholipids + H2O Gloydius halys presynaptic neurotoxicity of neutral PLA2 ?
-
 ?
3.1.1.4 phospholipids + H2O Gloydius halys cardiac toxicity ?
-
 ?
3.1.1.4 phospholipids + H2O Gloydius halys hemolytic activity of basic PLA2 ?
-
 ?
3.1.1.4 phospholipids + H2O Gloydius halys influence on platelet aggregation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Gloydius halys
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4
-
 Gloydius halys

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4 basic-acidic hybrid PLA2 Gloydius halys

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 venom
-
 Gloydius halys
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 500
-
 de-/renatured basic PLA2, PC for substrate Gloydius halys
3.1.1.4 650
-
 de-/renatured basic-acidic PLA2, PC for substrate Gloydius halys
3.1.1.4 750
-
 de-/renatured acidic PLA2, PA for substrate Gloydius halys
3.1.1.4 1500
-
 de-/renatured acidic PLA2, PC for substrate Gloydius halys
3.1.1.4 1600
-
 de-/renatured basic-acidic PLA2, PA for substrate Gloydius halys
3.1.1.4 1750
-
 de-/renatured basic PLA2, PA for substrate Gloydius halys

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 phosphatidylcholine + H2O
-
 Gloydius halys 1-acylglycerophosphocholine + fatty acid
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Gloydius halys lysophosphatidylcholine + a fatty acid
-
 ?
3.1.1.4 phospholipids + H2O myotoxicity Gloydius halys ?
-
 ?
3.1.1.4 phospholipids + H2O presynaptic neurotoxicity of neutral PLA2 Gloydius halys ?
-
 ?
3.1.1.4 phospholipids + H2O cardiac toxicity Gloydius halys ?
-
 ?
3.1.1.4 phospholipids + H2O hemolytic activity of basic PLA2 Gloydius halys ?
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Gloydius halys ?
-
 ?