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Literature summary extracted from

  • Momsen, W.E.; Brockman, H.L.
    Purification and characterization of cholesterol esterase from porcine pancreas (1977), Biochim. Biophys. Acta, 486, 103-113.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 diisopropylfluorophosphate complete inhibition above 0.1 mM Sus scrofa
3.1.1.13 p-mercuribenzoate 15% and 80% inhibition at 1 and 8.3 mM, partially reversed by addition of DTT Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 79000 82000 gel filtration, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Sus scrofa
-
 cholesterol + fatty acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13 94% purity, chromatography techniques Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 15.17
-
 for p-nitrophenyl acetate Sus scrofa
3.1.1.13 42.5
-
 for cholesteryl oleate Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 4-nitrophenyl acetate + H2O
-
 Sus scrofa 4-nitrophenol + acetate
-
 ?
3.1.1.13 cholesteryl esters + H2O
-
 Sus scrofa cholesterol + fatty acid
-
 ?
3.1.1.13 cholesteryl oleate + H2O
-
 Sus scrofa cholesterol + oleic acid
-
 ?
3.1.1.13 p-nitrophenyl acetate + H2O
-
 Sus scrofa p-nitrophenol + acetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 monomer 1 * 79000-82000, SDS-PAGE and gel filtration Sus scrofa
3.1.1.13 multimer complex of monomers Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.13 0.35
-
 cholesteryl oleate
-
 Sus scrofa
3.1.1.13 1.07
-
 4-nitrophenyl acetate
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 6.5 8.5
-
 Sus scrofa