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Literature summary extracted from
- Sterol ester hydrolase from rat pancreas (1981), Methods Enzymol., 71, 664-674.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.13 | cholic acid | other bile-salts not effective, form an enzymatically active hexamer | Rattus sp. |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.13 | more stable in aggregates than in monomers | Rattus sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 70000 | - |
SDS-PAGE | Rattus sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | Rattus sp. | - |
cholesterol + fatty acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | Rattus sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.13 | 500fold, standard chromatography techniques to homogeneity | Rattus sp. |
| 3.1.1.13 | digitonin-solubilized, affinity chromatography to homogeneity | Rattus sp. |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | pancreas | - |
Rattus sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 9.5 | - |
purified by affinity chromatography | Rattus sp. |
| 3.1.1.13 | 43.5 | - |
purified by standard chromatography techniques | Rattus sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.13 | -20°C, enzyme aggregates, 10 months | Rattus sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | - |
Rattus sp. | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl oleate + H2O | - |
Rattus sp. | cholesterol + oleic acid | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 6.2 | - |
for esterification | Rattus sp. |
| 3.1.1.13 | 6.6 | - |
for hydrolysis | Rattus sp. |
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Release 2023.1 (January 2023)
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