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Literature summary extracted from

  • Cook, K.G.; Colbran, R.J.; Snee, J.; Yeaman, S.J.
    Cytosolic cholesterol ester hydrolase from bovine corpus luteum. Its purification, identification, and relationship to hormone-sensitive lipase (1983), Biochim. Biophys. Acta, 752, 46-53.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 cAMP-dependent protein kinase type II 2fold activation in presence of ATP Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 diisopropylfluorophosphate 50% inhibition at 0.005 mM Bos taurus
3.1.1.13 Hg2+ 50% inhibition at 0.01 mM Bos taurus
3.1.1.13 NaF 50% inhibition at 3 mM Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.13 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 84000
-
 SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Bos taurus supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues cholesterol + fatty acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.13 side-chain modification phosphoprotein Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13 partial, 750fold, chromatography steps Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 corpus luteum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 0.34
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.1.13 -20°C, 5 mM sodium phosphate, 30% glycerol w/v, 0.04% Triton X-100 w/v, 15 mM 2-mercaptoethanol, pH 7.0, several weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues Bos taurus cholesterol + fatty acid
-
 ?
3.1.1.13 cholesteryl oleate + H2O
-
 Bos taurus cholesterol + oleic acid
-
 ?
3.1.1.13 monooleoylalkylglycerol + H2O
-
 Bos taurus alkylglycerol + oleic acid
-
 ?
3.1.1.13 triolein + H2O
-
 Bos taurus dioleoylglycerol + oleic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 monomer 1 * 84000, SDS-PAGE Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.13 6 8
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.1.13 ATP in presence of cAMP-dependent protein kinase Bos taurus